首都医药
首都醫藥
수도의약
CAPITAL MEDICINE
2014年
22期
110-111,112
,共3页
张小龙%孙晓%马新飞%孙思
張小龍%孫曉%馬新飛%孫思
장소룡%손효%마신비%손사
气相色谱法%小儿感冒颗粒%α-蒎烯%β-蒎烯%薄荷脑%百秋李醇
氣相色譜法%小兒感冒顆粒%α-蒎烯%β-蒎烯%薄荷腦%百鞦李醇
기상색보법%소인감모과립%α-파희%β-파희%박하뇌%백추리순
GC%Xiaoerganmao Granules%α-pinene%β-pinene%menthol%patchouli alcohol
目的：建立气相色谱法同时测定小儿感冒颗粒中百秋李醇、薄荷脑、α-蒎烯、β-蒎烯含量的方法。方法色谱柱：HP-5柱（30m×0.32mm×0.25μm）；柱温采用程序升温：起始40℃，保持5min，8℃/min升至180℃，保持2.5 min；进样口温度：250℃；检测器（FID）温度：300℃；载气为氮气，流速为2.0ml/min；进样量为1μl,分流比为20∶1。结果α-蒎烯在浓度为5.060-80.96μg/ml时线性关系良好，r=0.9999，平均回收率为101.75％，RSD为1.3％（n=6）；β-蒎烯在浓度为10.06~160.9μg/ml时线性关系良好，r=0.9997，平均回收率为102.26％，RSD为1.1％（n=6）；薄荷脑在浓度为5.035~80.56μg/ml时线性关系良好，r=0.9997，平均回收率为98.32％，RSD为0.8％（n=6）；百秋李醇在浓度为19.10~305.7μg/ml时线性关系良好，r=0.9998，平均回收率为99.69％， RSD为1.5％（n=6）。结论该方法简单、准确，能同时测定4种有效成分的含量，可作为小儿感冒颗粒的质量控制。
目的：建立氣相色譜法同時測定小兒感冒顆粒中百鞦李醇、薄荷腦、α-蒎烯、β-蒎烯含量的方法。方法色譜柱：HP-5柱（30m×0.32mm×0.25μm）；柱溫採用程序升溫：起始40℃，保持5min，8℃/min升至180℃，保持2.5 min；進樣口溫度：250℃；檢測器（FID）溫度：300℃；載氣為氮氣，流速為2.0ml/min；進樣量為1μl,分流比為20∶1。結果α-蒎烯在濃度為5.060-80.96μg/ml時線性關繫良好，r=0.9999，平均迴收率為101.75％，RSD為1.3％（n=6）；β-蒎烯在濃度為10.06~160.9μg/ml時線性關繫良好，r=0.9997，平均迴收率為102.26％，RSD為1.1％（n=6）；薄荷腦在濃度為5.035~80.56μg/ml時線性關繫良好，r=0.9997，平均迴收率為98.32％，RSD為0.8％（n=6）；百鞦李醇在濃度為19.10~305.7μg/ml時線性關繫良好，r=0.9998，平均迴收率為99.69％， RSD為1.5％（n=6）。結論該方法簡單、準確，能同時測定4種有效成分的含量，可作為小兒感冒顆粒的質量控製。
목적：건립기상색보법동시측정소인감모과립중백추리순、박하뇌、α-파희、β-파희함량적방법。방법색보주：HP-5주（30m×0.32mm×0.25μm）；주온채용정서승온：기시40℃，보지5min，8℃/min승지180℃，보지2.5 min；진양구온도：250℃；검측기（FID）온도：300℃；재기위담기，류속위2.0ml/min；진양량위1μl,분류비위20∶1。결과α-파희재농도위5.060-80.96μg/ml시선성관계량호，r=0.9999，평균회수솔위101.75％，RSD위1.3％（n=6）；β-파희재농도위10.06~160.9μg/ml시선성관계량호，r=0.9997，평균회수솔위102.26％，RSD위1.1％（n=6）；박하뇌재농도위5.035~80.56μg/ml시선성관계량호，r=0.9997，평균회수솔위98.32％，RSD위0.8％（n=6）；백추리순재농도위19.10~305.7μg/ml시선성관계량호，r=0.9998，평균회수솔위99.69％， RSD위1.5％（n=6）。결론해방법간단、준학，능동시측정4충유효성분적함량，가작위소인감모과립적질량공제。
Objective To establish a GC method for determination of patchouli alcohol,menthol,α-pinene,β-pinene in Xiaoerganmao Granules. Methods Column: HP-5 capillary column (30m×0.32mm×0.25μm);The increase of column temperature was controlled by programming:the initial temperature was 40℃ and maintained for 5 min,and then rose at 8℃/min up to 180℃ for 2.5min. The injector temperature was 250℃,the detector temperature was 300℃.The carrier gas was nitrogen, the flow rate was 2.0ml/min and the injection volumn was 1μl. The split ratio was 20∶1. Result The linear ranges of α-pinene,β-pinene, menthol and patchouli alcohol were at 5.060~80.96μg/ml (r=0.9999),10.06~160.9μg/ml (r=0.9997),5.035~80.56μg/ml (r=0.9997) and 19.10~305.7μg/ml (r=0.9998). The average recoveries (n=6) were 101.75%(RSD=1.3%, n=6),102.26% (RSD=1.1%, n=6), 98.32% (RSD=0.8%, n=6) and 99.69%(RSD=1.5%, n=6). Conclusion The method is simple and accurate. And it could determine four components of the composition simultaneously. The method can be used for quality control of Xiaoerganmao Granules.
