CAJ | 학술논문

目的：建立高效液相色谱（HPLC）法快速测定人血浆中万古霉素浓度的方法，监测ICU患者治疗药物的浓度。方法：色谱柱为Waters的Xbridge柱（4．6mm×150mm，5μm）；流动相为25mmol·L-1磷酸二氢钾-甲醇（86：14，pH=2.4），流速为1.0mL·min-1，紫外检测波长为236nm，进样量为20μL，柱温为40℃。结果：万古霉素浓度在1～100mg·L-1内线性关系良好（r=0.9992），定量下限为1.0mg·L-1，日内、日间精密度相对标准偏差（RSD）分别为5.83%和6.06%，提取回收率为79.71%。所监测的28例患者血样中，有12例患者首次万古霉素谷浓度在10～20mg·L-1之间，有17例治疗结果为有效，总有效率为60.7%。结论：HPLC法简便、灵敏、准确、快速、重复性好，可用于ICU患者万古霉素的血药浓度监测。万古霉素用药后血药浓度个体差异较大，临床需要监测结果，为ICU患者制定合理的个体化给药方案。
목적：건립고효액상색보（HPLC）법쾌속측정인혈장중만고매소농도적방법，감측ICU환자치료약물적농도。방법：색보주위Waters적Xbridge주（4．6mm×150mm，5μm）；류동상위25mmol·L-1린산이경갑-갑순（86：14，pH=2.4），류속위1.0mL·min-1，자외검측파장위236nm，진양량위20μL，주온위40℃。결과：만고매소농도재1～100mg·L-1내선성관계량호（r=0.9992），정량하한위1.0mg·L-1，일내、일간정밀도상대표준편차（RSD）분별위5.83%화6.06%，제취회수솔위79.71%。소감측적28례환자혈양중，유12례환자수차만고매소곡농도재10～20mg·L-1지간，유17례치료결과위유효，총유효솔위60.7%。결론：HPLC법간편、령민、준학、쾌속、중복성호，가용우ICU환자만고매소적혈약농도감측。만고매소용약후혈약농도개체차이교대，림상수요감측결과，위ICU환자제정합리적개체화급약방안。
Objective: To establish a HPLC method for fast determination of vancomycin in serum and using the method for monitoring ICU patients’ therapeutic drug concentration.Methods: HPLC analysis was performed using a Water’ Xbridge column (4.6 mm×150 mm, 5 μm). The mobile phase was KH2PO4(25 mmol?L-1)-methanol (86:14, pH=2.4). The lfow-rate was 1.0 mL?min-1 and the detection wave length was 236 nm by UV. The injection volume was 20 μL and the temperature of column was 40℃.Results: The concentration range of vancomycin was 1~100 mg?L-1 while the calibration curve showed good linearity (r=0.9992), and the minimum limit of quantitation was 1.0 mg?L-1. The relative standard deviations (RSD) of intra-day and inter-day were 5.83% and 6.06%, the extraction recovery was 79.71%. Twelve patients ifrst blood valley concentration of vancomycin was between 10 and 20 mg?L-1. There were 17 cases of the treatment for effective, total effective rate was 60.71%.Conclusion: The method is simple, sensitive, exact, rapid and good repeatability. It can be used for detecting the concentration of vancomycin in ICU patients. There is bigger individual difference in blood con-centration after giving Vancomycin. Clinical need monitoring results formulate reasonable individualized dosage regimen for ICU patients.