CAJ | 학술논문

目的：探讨Wilms肿瘤基因（WT1）在子宫内膜腺癌组织、子宫内膜不典型增生组织以及正常内膜组织中的蛋白和基因表达水平及其临床意义。方法：采用实时荧光定量PCR及2-ΔΔCt法定量分析WT1mRNA的相对表达量，应用蛋白印迹（Westernblot）法检测子宫内膜组织中WT1蛋白的表达。结果：WT1在子宫内膜腺癌组织中的基因和蛋白表达均明显高于子宫内膜不典型组织和正常内膜组织（均P＜0.01）；WT1mRNA与子宫内膜腺癌患者年龄、绝经情况、临床分期、淋巴结浸润情况、雌激素受体（ER）和体质量指数（BMI）无显著相关性，与病理分级、肌层浸润情况和孕激素受体（PR）相关（P＜0.05）；WT1蛋白的表达与患者年龄、绝经情况、临床分期、肌层浸润、ER、PR和BMI无显著相关性，与病理分级、淋巴结转移情况相关（P＜0.05）。结论：子宫内膜腺癌中存在WT1mRNA和蛋白表达水平上调，可能与子宫内膜腺癌的发生和进展有关。
목적：탐토Wilms종류기인（WT1）재자궁내막선암조직、자궁내막불전형증생조직이급정상내막조직중적단백화기인표체수평급기림상의의。방법：채용실시형광정량PCR급2-ΔΔCt법정량분석WT1mRNA적상대표체량，응용단백인적（Westernblot）법검측자궁내막조직중WT1단백적표체。결과：WT1재자궁내막선암조직중적기인화단백표체균명현고우자궁내막불전형조직화정상내막조직（균P＜0.01）；WT1mRNA여자궁내막선암환자년령、절경정황、림상분기、림파결침윤정황、자격소수체（ER）화체질량지수（BMI）무현저상관성，여병리분급、기층침윤정황화잉격소수체（PR）상관（P＜0.05）；WT1단백적표체여환자년령、절경정황、림상분기、기층침윤、ER、PR화BMI무현저상관성，여병리분급、림파결전이정황상관（P＜0.05）。결론：자궁내막선암중존재WT1mRNA화단백표체수평상조，가능여자궁내막선암적발생화진전유관。
Objective: To detect the protein and gene expression of WT1 in different types of tissues, in-cluding endometrial adenocarcinoma (EA) tissues, endometrial hyperplasia tissues and normal tissues and cor-related the expression with the clinicopathological features.Methods: The relative expression WT1 mRNA was detected by real-time quantitative PCR. The expression of WT1 protein was detected by Western blot in different types of endometrial tissues.Results: The expression of WT1 mRNA and protein in endometrial adenocarci-noma tissue was signiifcantly higher than that in endometrial hyperplasia tissues and normal endometrial tissue respectively (P<0.01). There was no signiifcant difference among age, menopause status, clinical stage, lymph node metastasis status, ER, BMI except histological stage, myometrial invasion and PR status concerned to WT1 gene expression (P<0.05). There were no signiifcant correlations among the protein expressionof WT1 and age, menopause status, clinical stage, myometrial invasion ER, PR, BMI (P>0.05), but related to histological stage and lymph node status, signiifcantly (P<0.05).Conclusion: Up-regulation of WT1 protein and gene expression are correlated to the oncogenesis and progression of EA.