CAJ | 학술논문

以中国明对虾为试验对象，优化了拥挤应激条件下中国明对虾肝胰脏蛋白质双向电泳的条件，并分析了关联的差异蛋白质。试验结果表明，中国明对虾肝胰脏蛋白双向电泳优化条件为裂解液中添加三丁基膦，采用17 cm的固相p H值（p H 4～7）梯度干胶条，上样量为120μg ，等电聚焦时间为65000 V／h。蛋白图谱分析结果显示，得到的700多个蛋白点中差异蛋白点11个，其中8个差异蛋白点丰度显著上调（P ＜0．05），3个差异蛋白点丰度显著下调（P ＜0．05）。研究表明，在拥挤应激条件下中国明对虾肝胰脏蛋白表达发生显著变化。
이중국명대하위시험대상，우화료옹제응격조건하중국명대하간이장단백질쌍향전영적조건，병분석료관련적차이단백질。시험결과표명，중국명대하간이장단백쌍향전영우화조건위렬해액중첨가삼정기련，채용17 cm적고상p H치（p H 4～7）제도간효조，상양량위120μg ，등전취초시간위65000 V／h。단백도보분석결과현시，득도적700다개단백점중차이단백점11개，기중8개차이단백점봉도현저상조（P ＜0．05），3개차이단백점봉도현저하조（P ＜0．05）。연구표명，재옹제응격조건하중국명대하간이장단백표체발생현저변화。
The two-dimensional electrophoresis (2-DE) was optimized and differentially expressed proteins were investigated in hepatopancreas of Chinese shrimp (Fenneropenaeus Chinensis) under crowded condi-tion .The results showed that the optimal two-dimensional electrophoresis were observed under the condi-tions of addition of TBP (tributyl phosphate) ,strips of 17 cm ,and pH 4-7 IPG (Immobilized pH gradi-ent) ,loading the sample of 120 μg and setting the voltage and power of isoelectric focusing as 65 000 Vhr . The relative 2-DE maps showed that approximate 600 spots was detected in each gel ,and a total of 11 dif-ferentially expressed protein spots were found ,including 8 significantly up-regulated protein spots (P <0 .05) and 3 significantly down-regulated protein spots (P < 0 .05) .The findings indicated that proteins in the shrimp hepatopancreas were differently expressed under crowding stress .