CAJ | 학술논문

将从平均体质量400 g的点带石斑鱼中肾分离出的白细胞培养在添加0、0．5、1、2 mmol／L 谷氨酰胺的培养基中2、6、12、24 h后，测定白细胞的吞噬和呼吸爆发活力，结果发现，添加谷氨酰胺后白细胞的吞噬和呼吸爆发活力显著提高，随着培养时间的延长，吞噬和呼吸爆发活力进一步提高，最高值均在培养12 h后的1 mmol／L组。在培养12、24 h后测定了白细胞的增殖能力，发现培养12 h后，添加谷氨酰胺的各组增殖效果明显优于未添加谷氨酰胺组（对照组）（ P＜0．05），但添加谷氨酰胺的各组之间差异不显著（P＞0．05），最高值在1 mmol／L组；培养48 h后，除2 mmol／L组外，其他处理组的增殖效果均下降。白细胞对迟钝爱德华氏菌杀菌率变化趋势与吞噬活力相符，1、2 mmol／L 组杀菌率显著高于对照组（P＜0．05），而0．5 mmol／L组与对照组之间差异不显著（P＞0．05）。试验结果表明，谷氨酰胺是石斑鱼有效的免疫增强剂，在培养基中添加1 mmol／L 谷氨酰胺培养12 h效果最佳。
장종평균체질량400 g적점대석반어중신분리출적백세포배양재첨가0、0．5、1、2 mmol／L 곡안선알적배양기중2、6、12、24 h후，측정백세포적탄서화호흡폭발활력，결과발현，첨가곡안선알후백세포적탄서화호흡폭발활력현저제고，수착배양시간적연장，탄서화호흡폭발활력진일보제고，최고치균재배양12 h후적1 mmol／L조。재배양12、24 h후측정료백세포적증식능력，발현배양12 h후，첨가곡안선알적각조증식효과명현우우미첨가곡안선알조（대조조）（ P＜0．05），단첨가곡안선알적각조지간차이불현저（P＞0．05），최고치재1 mmol／L조；배양48 h후，제2 mmol／L조외，기타처리조적증식효과균하강。백세포대지둔애덕화씨균살균솔변화추세여탄서활력상부，1、2 mmol／L 조살균솔현저고우대조조（P＜0．05），이0．5 mmol／L조여대조조지간차이불현저（P＞0．05）。시험결과표명，곡안선알시석반어유효적면역증강제，재배양기중첨가1 mmol／L 곡안선알배양12 h효과최가。
An ivitro experiment was conducted to evaluate the effects of glutamine (0 ,0 .5 ,1 ,2 mmol/L) on immune responses of kidney leukocytes in grouper (Epinephelus malabaricus) .After the leukocytes were cultured primarily in 2 ,6 ,12 ,24 h ,the phagocytosis and respiratory burst activity of leukocytes were determined ,respectively .Phagocytosis and respiratory burst activity of leukocytes were significantly enhanced with glutamine supplemented into the culture medium ,and increased further with the increasing culture time .The peak was occurred in 1 mmol/L glutamine group 12 h after incubation .The proliferation of leukocytes was determined 12 and 24 h after incubation in medium supplemented with glutamine .After 12 h incubation ,the proliferation effects in the groups supplemented with glutamine were significantly im-proved compared with the basal group ,and no significant differences were observed in groups supplemen-ted with glutamine ,as well as the highest proliferation activity was found in 1 mmol/L glutamine group . And the proliferation activity was decreased 24 h after incubation except 2 mmol/L glutamine group .The trend of killing ability against E .tarda in the leukocytes was similar with the phagocytosis .The killing a-bility of leukocytes incubated in medium supplemented in 1 and 2 mmol/L glutamine were significantly higher than that in basal group (P<0 .05) ,and no significant difference was observed in 0 .5 mmol/L glu-tamine group and the basal group (P>0 .05) .The findings indicate that glutamine is the effective immu-nomodulatory in grouper leukocytes ,and the optimum glutamine supplemental level 1 mmol/L at the incu-bation time of 12 h .