CAJ | 학술논문

目的：观察参附强心合剂对心衰大鼠心功能及水通道蛋白2（AQP-2）水平的影响，探讨参附强心合剂抗心衰水潴留的作用机制。方法用腹主动脉缩窄法制作心衰大鼠模型，造模成功60只大鼠随机分为参附强心合剂大、中、小剂量组，氯沙坦组和模型组，每组12只。前三组分别按成人用药剂量20、10、5倍的参附强心合剂14g/（kg·d）、7g/（kg·d）、3.5g/（kg·d）灌胃；氯沙坦组以成人剂量10倍的氯沙坦10mg/（kg·d）灌胃；模型组以蒸馏水灌胃。另假手术组大鼠12只以蒸馏水灌胃作为空白对照组。给药4周后，心超检查测定左室射血分数（LVEF），ELISA法检测大鼠尿AQP-2浓度，Western blot检测大鼠肾脏AQP-2表达水平。结果参附强心合剂可改善心衰大鼠心功能，其各剂量组大鼠尿AQP-2浓度及肾脏AQP-2表达水平均明显下降（P<0.01或P<0.05）；随参附强心合剂剂量的增加，AQP-2浓度逐渐下降，EF值逐渐升高；EF值与AQP-2间有良好的线性负性相关关系（P<0.01）。结论参附强心合剂可有效改善心衰大鼠心功能，降低AQP-2浓度，且呈一定的剂量依赖性；其作用机制与降低心衰大鼠AQP-2浓度有关。
목적：관찰삼부강심합제대심쇠대서심공능급수통도단백2（AQP-2）수평적영향，탐토삼부강심합제항심쇠수저류적작용궤제。방법용복주동맥축착법제작심쇠대서모형，조모성공60지대서수궤분위삼부강심합제대、중、소제량조，록사탄조화모형조，매조12지。전삼조분별안성인용약제량20、10、5배적삼부강심합제14g/（kg·d）、7g/（kg·d）、3.5g/（kg·d）관위；록사탄조이성인제량10배적록사탄10mg/（kg·d）관위；모형조이증류수관위。령가수술조대서12지이증류수관위작위공백대조조。급약4주후，심초검사측정좌실사혈분수（LVEF），ELISA법검측대서뇨AQP-2농도，Western blot검측대서신장AQP-2표체수평。결과삼부강심합제가개선심쇠대서심공능，기각제량조대서뇨AQP-2농도급신장AQP-2표체수평균명현하강（P<0.01혹P<0.05）；수삼부강심합제제량적증가，AQP-2농도축점하강，EF치축점승고；EF치여AQP-2간유량호적선성부성상관관계（P<0.01）。결론삼부강심합제가유효개선심쇠대서심공능，강저AQP-2농도，차정일정적제량의뢰성；기작용궤제여강저심쇠대서AQP-2농도유관。
Objective To investigate the effect of Shenfu qiangxin(SFQX) decoction on cardiac function and the level of urinary aquaporin 2 in chronic heart failure (CHF) rat model, in an attempt to explore the underlying mechanism of SFQX decoction on water retention in CHF. Methods Sixty CHF models in male adult SD rats were made by coarctation of abdominal aorta (CAA) and then were randomly divided into 5 groups: SFOX groups (large, medium, and low dose), losarton group, and model group. Another 12 SD rats received sham operation. Large-, medium-, and low-dose SFQX groups were intragastically given 14, 7 and 3.5 g·kg-1·d-1 SFQX decoction, losarton group was given 10 mg·kg-1·d-1 of losarton, and model group and sham group were grive 2 mL/d of sterile purified water. At the end of 4 weeks, the cardiac function was measured by ultrasonic echocardiogram, and AQP-2 was detected by ELISA and western blot. Results Comparing with model group, the cardiac function were im-proved in SFQX groups;the concentration of urinary AQP-2 and the expression of AQP-2 in renal tissue were re-duced(P<0.01 or P<0.05). The concentration of AQP-2 reduced but ejection fraction(EF) increased correspondingly with the increase of the dose of SFQX decoction. There was a remarkable negative correlation between EF and the concentration of urinary AQP-2 (P<0.01). Conclusion SFQX decoction can improve the cardiac function and re-duce the concentration of AQP-2 in CHF rat model in a dose-dependent manner. The mechanism of SFQX decoc-tion on water retention in CHF rats may be related to reduce the concentration of AQP-2.