粮油加工（电子版）
糧油加工（電子版）
량유가공（전자판）
Cereals and Oils Processing（Electronic Version）
2014年
11期
52-54
,共3页
黄秋研%綦艳%郦明浩
黃鞦研%綦豔%酈明浩
황추연%기염%역명호
维生素E组分%正相高效液相色谱%花生油
維生素E組分%正相高效液相色譜%花生油
유생소E조분%정상고효액상색보%화생유
Vitamin E components%Normal-phase high performance liquid chromatography%Peanut oil
建立正相高效液相色谱法测定花生油中的维生素E组分的方法。花生油中维生素E用正己烷直接提取后采用正相高效液相色谱法，荧光检测器检测。维生素E各组分浓度在0.01～100g/mL峰面积与含量具有良好的线性关系，平均相对标准偏差在0.47%~0.92%，平均回收率为94.9%~98.7%。该方法快速，准确，灵敏度高，可作为花生油中维生素E含量测定的检测方法。
建立正相高效液相色譜法測定花生油中的維生素E組分的方法。花生油中維生素E用正己烷直接提取後採用正相高效液相色譜法，熒光檢測器檢測。維生素E各組分濃度在0.01～100g/mL峰麵積與含量具有良好的線性關繫，平均相對標準偏差在0.47%~0.92%，平均迴收率為94.9%~98.7%。該方法快速，準確，靈敏度高，可作為花生油中維生素E含量測定的檢測方法。
건립정상고효액상색보법측정화생유중적유생소E조분적방법。화생유중유생소E용정기완직접제취후채용정상고효액상색보법，형광검측기검측。유생소E각조분농도재0.01～100g/mL봉면적여함량구유량호적선성관계，평균상대표준편차재0.47%~0.92%，평균회수솔위94.9%~98.7%。해방법쾌속，준학，령민도고，가작위화생유중유생소E함량측정적검측방법。
A normal-phase high performance liquid chromatography method was developed for determination of vitamin E components in peanut oil. Vitamin E components in peanut oil samples were extracted by n-hexane, and then determined by normal-phase high performance liquid chromatography with fluorescence detection. Results showed that 0.01~100 g/mL vitamin E components and peak areas were of good linear relationship. Average relative standard deviations were 0.47%~0.92%and average spike recoveries were 94.9%~98.7%. This method demonstrated to be simple and accu-rate with high precision, and thus had a promising potential for the determination of vitamin E in peanut oil.
