CAJ | 학술논문

目的：研究金茵利胆灵对豚鼠胆囊容积和成石率与G蛋白偶联胆汁酸受体表达的影响。方法取雌性豚鼠72只，随机分为6组，每组12只，除正常对照组外其余5组给予盐酸林可霉素皮下注射建立胆石症模型。治疗组分为4组给予阿司匹林，金茵利胆灵高、中、低剂量药液灌喂，末次给药后1 h，切除胆囊测量胆囊容积，计算每组动物的胆囊内成石率。将胆囊组织进行HE染色后行显微镜观察，并行免疫组化观察阳性细胞。多组间比较采用单因素方差分析，两组间比较用LSD－t法；计数资料采用χ2检验，两组间比较调整检验水准进行比较；等级资料采用Kruskal－Wallis H检验。结果模型组成石率高于正常对照组，差异有统计学意义（P＝0．00）；金茵利胆灵高剂量组成石率较其他组降低。模型组胆囊大量中性粒细胞浸润，炎性改变明显；各药物治疗组中金茵高剂量组炎性改变最轻。模型组胆囊容积增加明显，与正常对照组相比差异有统计学意义（P＜0．01），造模效果较好；金茵利胆灵高剂量组胆囊容积相对最小，金茵利胆灵中剂量组和阿司匹林组胆囊容积相对略小，与模型组相比差异均有统计学意义（P＜0.01）。模型组胆囊黏膜TGR5阳性表达强，与正常对照组相比差异有统计学意义（P＝0．00）；金茵利胆灵高剂量组黏膜TGR5阳性表达较弱，与模型组相比差异有统计学意义（P＜0．01）。结论 TGR5在胆石症豚鼠胆囊黏膜的表达显著增强。金茵利胆灵可以降低胆石症豚鼠胆囊黏膜TGR5阳性表达率，可减轻胆囊炎症，减少胆石形成。
목적：연구금인리담령대돈서담낭용적화성석솔여G단백우련담즙산수체표체적영향。방법취자성돈서72지，수궤분위6조，매조12지，제정상대조조외기여5조급여염산림가매소피하주사건립담석증모형。치료조분위4조급여아사필림，금인리담령고、중、저제량약액관위，말차급약후1 h，절제담낭측량담낭용적，계산매조동물적담낭내성석솔。장담낭조직진행HE염색후행현미경관찰，병행면역조화관찰양성세포。다조간비교채용단인소방차분석，량조간비교용LSD－t법；계수자료채용χ2검험，량조간비교조정검험수준진행비교；등급자료채용Kruskal－Wallis H검험。결과모형조성석솔고우정상대조조，차이유통계학의의（P＝0．00）；금인리담령고제량조성석솔교기타조강저。모형조담낭대량중성립세포침윤，염성개변명현；각약물치료조중금인고제량조염성개변최경。모형조담낭용적증가명현，여정상대조조상비차이유통계학의의（P＜0．01），조모효과교호；금인리담령고제량조담낭용적상대최소，금인리담령중제량조화아사필림조담낭용적상대략소，여모형조상비차이균유통계학의의（P＜0.01）。모형조담낭점막TGR5양성표체강，여정상대조조상비차이유통계학의의（P＝0．00）；금인리담령고제량조점막TGR5양성표체교약，여모형조상비차이유통계학의의（P＜0．01）。결론 TGR5재담석증돈서담낭점막적표체현저증강。금인리담령가이강저담석증돈서담낭점막TGR5양성표체솔，가감경담낭염증，감소담석형성。
Objective To investigate the effects of Jinyinlidanling (JYLDL)on gallbladder volume,rate of gallstone formation,and the ex-pression of G protein-coupled bile acid receptor (TGR5 )in guinea pigs.Methods Seventy-two female guinea pigs were randomly divid-ed into treatment groups,normal control group,and model group,with 12 ones in each group.Guinea pigs in the treatment groups and mod-el group were subcutaneously injected with lincomycin hydrochloride to build a cholelithiasis model,while the normal control group with the same volume of normal saline.The treatment groups were divided into four groups which were separately given aspirin and low-,medium-and high-dose JYLDL.At one hour after the last administration,the gallbladder of each guinea pig was resected to measure the gallbladder volume and calculate the rate of gallstone formation.HE staining was applied for microscopic observation,and positive cells (with expression of TGR5 )were detected by immunohistochemistry.Comparison between multiple groups was made by one-way ANOVA,and comparison between two groups by LSD-t test;comparison of categorical data was made by chi-square test,and the significance level was adjusted when comparison was made between two groups;comparison of ranked data was made by Kruskal Wallis H test.Results The rate of gall-stone formation in the model group was significantly higher than that in the control group (P=0.00);the rate of gallstone formation in the high-dose JYLDL group was lower than those of other groups.Typical inflammatory changes and infiltration of neutrophils were observed in the model group;the high-dose JYLDL group had the mildest inflammatory changes among treatment groups.The gallbladder volume of the model group was significantly bigger than that of the control group (P<0.01),indicating that the cholelithiasis model was successfully es-tablished;the gallbladder volume of the high-dose JYLDL group was the smallest and those of the medium-dose JYLDL group and aspirin group were smaller,as compared with the model group (P<0.01 ).The expression of TGR5 in the gallbladder mucosa was significantly higher in the model group than in the normal control group (P=0.00),while the high-dose JYLDL group had a significantly lower expres-sion of TGR5 than the model group (P<0.01).Conclusion The expression of TGR5 in the gallbladder mucosa is significantly increased in guinea pigs with cholelithiasis.JYLDL can reduce the positive rate of TGR5 expression in the gallbladder mucosa,the inflammation of the gallbladder,and the formation of gallstones among guinea pigs with cholelithiasis.