CAJ | 학술논문

在单因素实验的基础上,采用Box-Behnken中心组合实验和响应面分析法,研究提取时间、超声波功率、液料比对垂丝海棠叶多糖含量的影响,建立影响因素与响应值之间的数学模型,确立最佳提取工艺为：提取时间20 min,液料比45∶1,超声波功率135W。抗氧化实验结果表明,在达到最大浓度0．74mg·mL-1时,垂丝海棠叶多糖(超声)、垂丝海棠叶多糖(煮沸)和Vc的对DPPH的清除率依次为79．9%,69．7%,64．8%,垂丝海棠叶多糖(超声)和垂丝海棠叶多糖(煮沸)对·OH自由基的清除率分别为60．1%、51．2%,垂丝海棠叶多糖(超声)还原Fe3+能力较强。
재단인소실험적기출상,채용Box-Behnken중심조합실험화향응면분석법,연구제취시간、초성파공솔、액료비대수사해당협다당함량적영향,건립영향인소여향응치지간적수학모형,학립최가제취공예위：제취시간20 min,액료비45∶1,초성파공솔135W。항양화실험결과표명,재체도최대농도0．74mg·mL-1시,수사해당협다당(초성)、수사해당협다당(자비)화Vc적대DPPH적청제솔의차위79．9%,69．7%,64．8%,수사해당협다당(초성)화수사해당협다당(자비)대·OH자유기적청제솔분별위60．1%、51．2%,수사해당협다당(초성)환원Fe3+능력교강。
According to single-factor experiments,Box-Behnken design and its response surface analysis,main factors that affectaed the polysaccharides yield from the leaves in Malus halliana koehne such as extraction time,ultrasonic power,liquid-material ratio were studied to find the optimal process. The best extraction process was as follows:liquid-material ratio was 45 ∶ 1,extracting 20 min by 135W power of ultrasound. According to the results of antioxidant activities,the DPPH scavenging activities of polysaccha-rides extracted by ultrasound,polysaccharides extracted by boiling method and vitamin C was respectively 79. 9%,69. 7%,64. 8%when concentration of the polysaccharides reached the 0. 74mg·mL-1 . Scavenging activities of free radical·OH of the polysaccha-rides extracted by ultrasound and boiling method was respectively 60. 1% and 51. 2%. Meanwhile,the polysaccharides from ultra-sonic extraction revealed their better Fe3+ reduction activities.