CAJ | 학술논문

通过反相悬浮聚合法制备了超顺磁性环氧聚合物微球用于固定化青霉素G酰化酶,利用磁性固定化酶催化N-苯乙酰-( R,S)-2-氯苯甘氨酸进行不对称水解反应,制备出( S)-2-氯苯甘氨酸单一对映体。磁性固定化酶催化水解反应的适宜条件为：底物浓度100 mg·mL-1,反应温度和时间30℃和12 h,反应溶液pH 8．0。在此条件下,N-苯乙酰-(R,S)-2-氯苯甘氨酸的转化率为48．8%,产物(S)-2-氯苯甘氨酸的对映体过量值eep达99．4%。磁场下回收磁性固定化青霉素G酰化酶,重复使用6次,底物的转化率和产物的对映体过量值分别为47．8%和91．4%。
통과반상현부취합법제비료초순자성배양취합물미구용우고정화청매소G선화매,이용자성고정화매최화N-분을선-( R,S)-2-록분감안산진행불대칭수해반응,제비출( S)-2-록분감안산단일대영체。자성고정화매최화수해반응적괄의조건위：저물농도100 mg·mL-1,반응온도화시간30℃화12 h,반응용액pH 8．0。재차조건하,N-분을선-(R,S)-2-록분감안산적전화솔위48．8%,산물(S)-2-록분감안산적대영체과량치eep체99．4%。자장하회수자성고정화청매소G선화매,중복사용6차,저물적전화솔화산물적대영체과량치분별위47．8%화91．4%。
The prepared polymer microspheres with epoxy groups were super paramagnetic by inverse suspension polymerization for immobilizing penicillin G acylase. ( S)-2-chlorophenylglycine was synthesized via asymmetric hydrolysis of N-phenylacetic-( R,S)-2-chlorophenylglycine using the magnetic immobilized penicillin G acylase. Under the optimum reaction conditions of the substrate concentration 100 mg·mL-1,temperature 30℃,pH 8. 0,reaction time 12 h,the conversion of N-phenylacetic-(R,S)-2-chloropheny-lglycine was 48. 8%,the enantiomeric excess( eep ) of( S)-2-chlorophenylglycine was 99. 4%. The conversion of substrate and enan-tiomeric excess( eep ) of product was 47. 8%,and 91. 4%respectively by recovering the immobilized penicillin G acylase in magnetic field in six recycling tests.