CAJ | 학술논문

通过对甘肃省部分地区奶牛场的323份乳腺炎乳样进行金黄色葡萄球菌的分离及对分离株进行耐药性分析，旨在为临床合理用药提供科学依据。采用血平板培养基、选择性培养基（Baird-Parker培养基），营养琼脂培养基，结合革兰染色、镜检，对可疑菌落分离纯化后进行血浆凝固酶试验，确定分离株是否为金黄色葡萄球菌；选用临床常用的10种抗生素对分离株进行药敏试验。结果表明，170株分离株对青霉素、头孢曲松钠、四环素、克林霉素表现出明显耐药，耐药率分别为75.29%、60.59%、63.53%、61.76%，而对左氧氟沙星、环丙沙星、氯霉素高度敏感，敏感率分别为72.94%、74.71%、71.76%。
통과대감숙성부분지구내우장적323빈유선염유양진행금황색포도구균적분리급대분리주진행내약성분석，지재위림상합리용약제공과학의거。채용혈평판배양기、선택성배양기（Baird-Parker배양기），영양경지배양기，결합혁란염색、경검，대가의균락분리순화후진행혈장응고매시험，학정분리주시부위금황색포도구균；선용림상상용적10충항생소대분리주진행약민시험。결과표명，170주분리주대청매소、두포곡송납、사배소、극림매소표현출명현내약，내약솔분별위75.29%、60.59%、63.53%、61.76%，이대좌양불사성、배병사성、록매소고도민감，민감솔분별위72.94%、74.71%、71.76%。
Through isolation, identiifcation and drug resistance analysis of Staphylococcusaureusfrom 323 mastitis dairy cows in some region in Gansu to provide a scientiifc basis for rational clinical use of drug. Staphylococcus aureus was separated and purified by using Baird-Parker medium,Common nutrient AGAR plate,nutrient agar medium and microscope detection technology. Staphylococcus aureus were identificatied by plasma coagulase test. Totally 10 kinds of frequently-used antibiotics from the different dairy farm in Gansu were used in drug sensitive test. Result of antibiotic susceptibility testing indicated that 170 strains of Staphylococcus aureus were highly drugfast to penicillin, ceftriaxone sodium, tetracycline and clindamycin.The drug resistance rate respectively were 75.29%, 60.59%, 63.53% and 61.76%. By contrast, Quinolones levolfoxacin, ciprolfoxacin and chloramphenicol were high antibiotic susceptible rate were 72.94%、74.70%and 71.76%.