国际生殖健康/计划生育杂志
國際生殖健康/計劃生育雜誌
국제생식건강/계화생육잡지
JOURNLA OF INTERNATIONAL REPRODUCTIVE HEALTH/FAMILY PLANNING
2014年
4期
274-276
,共3页
胚胎冷冻%冻融胚胎移植%复苏率%妊娠率
胚胎冷凍%凍融胚胎移植%複囌率%妊娠率
배태냉동%동융배태이식%복소솔%임신솔
Embryo cryopreservation%Frozen-thawed embryo transfer%Survival rate%Pregnancy rate
目的：探讨胚胎卵裂球数及取卵后2天（D2）和3天（D3）冷冻对胚胎的复苏效果以及冻融胚胎移植（FET）临床结局的影响。方法：回顾性分析2010年1月-2012年5月行FET的417个周期的临床资料，分别按胚胎冷冻的时间（D2/D3）、卵裂球数、移植优质胚胎数进行分组，比较各组的胚胎复苏效果及临床结局。结果：D2≥4-细胞组的胚胎复苏率和卵裂球全存活率显著高于D2＜4-细胞组（89.35%vs.79.11%，42.01% vs.30.22%，P＜0.05）；D3≥6-细胞组的胚胎复苏率和卵裂球全存活率显著高于D3＜6-细胞组（86.52%vs.72.43%，41.13%vs.31.07%，P＜0.01）。D2组冷冻胚胎复苏后的优质胚胎率（56.84%）高于D3组（48.82%），但D3组FET临床妊娠率（38.52%）高于D2组（27.61%），差异均有统计学意义（P＜0.05），2组间胚胎复苏率和流产率差异无统计学意义（83.50%vs.79.44%，10.81%vs.10.09%，P＞0.05）。随着移植优质胚胎数的增加，临床妊娠率和多胎率增加，差异有统计学意义（P＜0.05）。结论：D2≥4-细胞和D3≥6-细胞的胚胎冻融效果较好，D3冷冻胚胎的FET临床结局优于D2冷冻胚胎。
目的：探討胚胎卵裂毬數及取卵後2天（D2）和3天（D3）冷凍對胚胎的複囌效果以及凍融胚胎移植（FET）臨床結跼的影響。方法：迴顧性分析2010年1月-2012年5月行FET的417箇週期的臨床資料，分彆按胚胎冷凍的時間（D2/D3）、卵裂毬數、移植優質胚胎數進行分組，比較各組的胚胎複囌效果及臨床結跼。結果：D2≥4-細胞組的胚胎複囌率和卵裂毬全存活率顯著高于D2＜4-細胞組（89.35%vs.79.11%，42.01% vs.30.22%，P＜0.05）；D3≥6-細胞組的胚胎複囌率和卵裂毬全存活率顯著高于D3＜6-細胞組（86.52%vs.72.43%，41.13%vs.31.07%，P＜0.01）。D2組冷凍胚胎複囌後的優質胚胎率（56.84%）高于D3組（48.82%），但D3組FET臨床妊娠率（38.52%）高于D2組（27.61%），差異均有統計學意義（P＜0.05），2組間胚胎複囌率和流產率差異無統計學意義（83.50%vs.79.44%，10.81%vs.10.09%，P＞0.05）。隨著移植優質胚胎數的增加，臨床妊娠率和多胎率增加，差異有統計學意義（P＜0.05）。結論：D2≥4-細胞和D3≥6-細胞的胚胎凍融效果較好，D3冷凍胚胎的FET臨床結跼優于D2冷凍胚胎。
목적：탐토배태란렬구수급취란후2천（D2）화3천（D3）냉동대배태적복소효과이급동융배태이식（FET）림상결국적영향。방법：회고성분석2010년1월-2012년5월행FET적417개주기적림상자료，분별안배태냉동적시간（D2/D3）、란렬구수、이식우질배태수진행분조，비교각조적배태복소효과급림상결국。결과：D2≥4-세포조적배태복소솔화란렬구전존활솔현저고우D2＜4-세포조（89.35%vs.79.11%，42.01% vs.30.22%，P＜0.05）；D3≥6-세포조적배태복소솔화란렬구전존활솔현저고우D3＜6-세포조（86.52%vs.72.43%，41.13%vs.31.07%，P＜0.01）。D2조냉동배태복소후적우질배태솔（56.84%）고우D3조（48.82%），단D3조FET림상임신솔（38.52%）고우D2조（27.61%），차이균유통계학의의（P＜0.05），2조간배태복소솔화유산솔차이무통계학의의（83.50%vs.79.44%，10.81%vs.10.09%，P＞0.05）。수착이식우질배태수적증가，림상임신솔화다태솔증가，차이유통계학의의（P＜0.05）。결론：D2≥4-세포화D3≥6-세포적배태동융효과교호，D3냉동배태적FET림상결국우우D2냉동배태。
Objective: To explore the impact of embryo blastomere number at cryopreservation, and different cryopreservation time on day 2 (D2) or day 3 (D3) after in vitro fertilization,on the clinical outcomes of the frozen-thawed embryo transfer (FET). Methods:This is a retrospective analysis on 417 FET cycles. The patients were respectively divided into different groups according to cryopreservation time after in vitro fertilization,embryo blastomere number and good-quality embryo number. The clinical outcomes of the embryo-transfer were compared. Results:The embryo survival rate and total blastomere survival rate in the group of the blastomere number exceeded 4-cells on D2 after frozen-thawed were significantly higher than those in the group of the blastomere number less than 4-cells(89.35%vs. 79.11%,42.01%vs. 30.22%respectively,P<0.05). Compared with the group of blastomere less than 6-cells on D3,the group of blastomere exceeded 6-cells on D3 had higher embryo survival rate and total blastomere survival rate(86.52%vs. 72.43%,41.13%vs. 31.07%, P<0.01). The good-quality embryo rate on D2(56.84%) was significantly higher than that on D3 (48.82%), while the clinical pregnancy rate on D3(38.52%) was significantly higher than that of D2 (27.61%). There was no significant difference in the embryo survival rate and abortion rate between D2 and D3 (83.50%vs. 79.44%, 10.81% vs. 10.09% ,P>0.05) after frozen-thawed embryo-transfer. More good-quality embryos were transferred,higher the clinical pregnancy rate and multiple pregnancy rate obtained(P<0.05). Conclusions:Cryopreservation of more than 4 blastomeres on D2 and more than 6 blastomeres on D3 could get better frozen-thawed effect. The clinical outcomes of D3 cryopreservation embryo after FET were superior to D2 embryo.
