CAJ | 학술논문

目的：探讨对实验鼠卵母细胞染色体C染色的简易方法。方法：分别采用染色体C带吉姆萨染色方法和改良的染色体C带吉姆萨染色方法后，对实验鼠卵母细胞直接采用4，6-二脒基-2-苯基吲哚（DAPI）进行染色体C带荧光染色法，通过荧光显微镜观察染色体的形态与数量。结果：染色体C带吉姆萨染色方法的染色弱致使染色体分辨率低，改良后的染色体C带吉姆萨染色法易导致染色体溶解，而染色体C带荧光染色法不仅增加了染色体着丝粒和淡染的染色体臂的对比度，而且缩短了染色时间。结论：染色体C带荧光染色法是鉴别实验鼠卵细胞正常染色体与非整倍性染色体的简易方法。
목적：탐토대실험서란모세포염색체C염색적간역방법。방법：분별채용염색체C대길모살염색방법화개량적염색체C대길모살염색방법후，대실험서란모세포직접채용4，6-이미기-2-분기신타（DAPI）진행염색체C대형광염색법，통과형광현미경관찰염색체적형태여수량。결과：염색체C대길모살염색방법적염색약치사염색체분변솔저，개량후적염색체C대길모살염색법역도치염색체용해，이염색체C대형광염색법불부증가료염색체착사립화담염적염색체비적대비도，이차축단료염색시간。결론：염색체C대형광염색법시감별실험서란세포정상염색체여비정배성염색체적간역방법。
Objective:To investigate the oocyte aneuploid in mice with the method of chromosome C-banding staining. Methods:The standard Giemsa C-banding staining and the modified Giemsa C-banding staining were used to show mouse chromosomes, respectively. The DAPI (4′, 6-diamidino-2-phenylindole) staining, so-called fluorescence C-banding staining, was used to show oocytes. Subsequently, the morphology and number of chromosomes were analyzed with fluorescence microscope. Results:Chromosomes were weakly stained by the standard Giemsa C-banding staining, while chromosomes were easily dissolved by the modified Giemsa C-banding staining. The fluorescence C-banding staining showed superiorities, such as the increased contrast degree between the brilliant white centromeres and the strong blue arms of chromosomes, and the shorten dyeing time. Conclusions:The study suggested that the fluorescence C-banding staining was a simple and rapid method to identify aneuploidy of mouse oocytes.