CAJ | 학술논문

目的：研究大针手法针刺督脉对癫痫大鼠海马神经细胞核转录因子κB(nuclear factor kappaB,NF κB)表达的影响,探讨大针手法针刺督脉治疗癫痫的作用机制。方法30只健康 Wistar 大鼠,体重200 g~250 g,雌雄各半,随机分为空白对照组(A组)、癫痫模型组(B 组)、大针手法组(C 组),每组10只,雌雄各半,癫痫模型组和大针手法组大鼠腹腔注射氯化锂(3 mmol/kg)和毛果芸香碱(匹鲁卡品,30 mg/kg,分3次注射),诱导癫痫持续状态(status epilepticus,SE)大鼠模型,观察大鼠的行为学改变,空白对照组腹腔注射同等剂量的生理盐水代替氯化锂和匹鲁卡品。空白对照组和癫痫模型组不做任何治疗,大针手法组用1寸不锈钢针,针刺大鼠督脉穴：风府、大椎、陶道、无名、身柱,每日1次,每次20 min,中间行针一次,平补平泻法,治疗14 d。运用 HE 染色法观察3组大鼠海马神经细胞病理形态学改变,运用免疫组化(SABC)法观测3组大鼠海马神经 NF κB P65阳性细胞数的表达。结果癫痫模型组和大针手法组大鼠均诱导出癫痫持续状态,两组各死亡1只。HE 染色显示,癫痫模型组大鼠海马神经细胞出现明显的病理改变,大针手法组大鼠海马神经细胞病理改变明显改善,空白对照组大鼠神经细胞未见明显病理改变。免疫组化染色：3组大鼠海马神经 NF κB P65阳性细胞数的表达依次为(13.95±6.99)个、(28.75±8.80)个、(19.25±8.60)个,癫痫模型组、大针手法组与空白对照组比较,差异有统计学意义(P <0.05),大针手法组与癫痫模型组比较,差异有统计学意义(P <0.05)。结论大针针刺督脉可能是通过抑制癫痫大鼠海马神经细胞 NF κB 的表达,从而减少海马神经细胞的凋亡,达到治疗癫痫的目的。
목적：연구대침수법침자독맥대전간대서해마신경세포핵전록인자κB(nuclear factor kappaB,NF κB)표체적영향,탐토대침수법침자독맥치료전간적작용궤제。방법30지건강 Wistar 대서,체중200 g~250 g,자웅각반,수궤분위공백대조조(A조)、전간모형조(B 조)、대침수법조(C 조),매조10지,자웅각반,전간모형조화대침수법조대서복강주사록화리(3 mmol/kg)화모과예향감(필로잡품,30 mg/kg,분3차주사),유도전간지속상태(status epilepticus,SE)대서모형,관찰대서적행위학개변,공백대조조복강주사동등제량적생리염수대체록화리화필로잡품。공백대조조화전간모형조불주임하치료,대침수법조용1촌불수강침,침자대서독맥혈：풍부、대추、도도、무명、신주,매일1차,매차20 min,중간행침일차,평보평사법,치료14 d。운용 HE 염색법관찰3조대서해마신경세포병리형태학개변,운용면역조화(SABC)법관측3조대서해마신경 NF κB P65양성세포수적표체。결과전간모형조화대침수법조대서균유도출전간지속상태,량조각사망1지。HE 염색현시,전간모형조대서해마신경세포출현명현적병리개변,대침수법조대서해마신경세포병리개변명현개선,공백대조조대서신경세포미견명현병리개변。면역조화염색：3조대서해마신경 NF κB P65양성세포수적표체의차위(13.95±6.99)개、(28.75±8.80)개、(19.25±8.60)개,전간모형조、대침수법조여공백대조조비교,차이유통계학의의(P <0.05),대침수법조여전간모형조비교,차이유통계학의의(P <0.05)。결론대침침자독맥가능시통과억제전간대서해마신경세포 NF κB 적표체,종이감소해마신경세포적조망,체도치료전간적목적。
Objective To study the effects of nuclear factor kappa B(NF κB)expression in hippocampal nerve cells of epileptic rats through dazhen acupuncture manipulation on Du Meridian,then explore the mechanism of anti epilepsy through dazhen acupunc-ture manipulation on Du Meridian,provide new theoretical basis for clinical acupuncture treatment of epilepsy.Methods Thirty health rats were randomly and enenly divided into blank control group(group A),epilepsy model group(group B),dazhen acupuncture manipulation group(group C),Lithium chloride and pilocarpine were injected the rats’enterocoelias of group B and group C,induce status epilepticus(SE)model.The rats’behavior changes were observed.Equal dose of normal saline were injected the rats’entero-coelia of group A.Any treatment was not given to group A,dazhen acupuncture manipulation was applied at points of Du Meridian :Fengfu,Dazhui,Taodao,Wuming,Shenzhu,once a day,20 minutes every time,treatment for 14 days.The pathological morphological changes in the hippocampal nerve cells of the three groups were observed by using HE staining.The expression of NF κB P65 in hip-pocampal nerve cells of the epileptic rats were observed by immunohistochemistry.Results The status epilepticus were induced in group B and group C,one rat dead in two groups.The rats in group B had obvious pathological morphological changes,the pathologi-cal morphological change in group C had obvious improvement.There was no obvious pathological morphological changes in group A.Immunohistochemical staining:The expression of NF κB P65 was(13.95±6.99)in group A,(28.75±8.80)in group B,(19.25± 8.60)in group C.Comparing each two groups,the differences are statistically significant(P <0.05).Conclusion Dazhen acupuncture manipulation on Du meridian,maybe can suppress the expression of NF κB P65 in the hippocampal nerve cells of epileptic rats,then reduce the hippocampal nerve cells apoptosis,treat epilepsy.