CAJ | 학술논문

目的：观察补肾活血方对血管性认知障碍模型大鼠海马组织神经递质及其受体表达的影响。方法3月龄 Wistar 大鼠40只,采用双侧颈总动脉永久结扎法(2 VO)制作认知障碍模型大鼠,分别给予补肾活血方和银杏叶片15 d。运用高效液相色谱法检测大鼠海马组织内乙酰胆碱(ACh)、5羟色胺(5 HT)、多巴胺(DA)、谷氨酸(Glu)、甘氨酸(Gly)、γ氨基丁酸(GABA)含量,免疫组化法检测其相应受体表达情况。结果与模型组相比,补肾活血方组中大鼠海马组织内 ACh、5 HT、DA、GABA 的含量及神经元中相应受体的表达均增高(P <0.05或P <0.01),Glu、Gly的含量及神经元中相应受体的表达均降低(P <0.05或P <0.01)。结论补肾活血方可减轻神经元损伤导致的脑内 ACh、5 HT、DA 含量的减少,保护大脑学习记忆能力；其机制可能与其调节血管性认知障碍模型大鼠脑内兴奋性氨基酸(EAA)/抑制性氨基酸(IAA)平衡有关。
목적：관찰보신활혈방대혈관성인지장애모형대서해마조직신경체질급기수체표체적영향。방법3월령 Wistar 대서40지,채용쌍측경총동맥영구결찰법(2 VO)제작인지장애모형대서,분별급여보신활혈방화은행협편15 d。운용고효액상색보법검측대서해마조직내을선담감(ACh)、5간색알(5 HT)、다파알(DA)、곡안산(Glu)、감안산(Gly)、γ안기정산(GABA)함량,면역조화법검측기상응수체표체정황。결과여모형조상비,보신활혈방조중대서해마조직내 ACh、5 HT、DA、GABA 적함량급신경원중상응수체적표체균증고(P <0.05혹P <0.01),Glu、Gly적함량급신경원중상응수체적표체균강저(P <0.05혹P <0.01)。결론보신활혈방가감경신경원손상도치적뇌내 ACh、5 HT、DA 함량적감소,보호대뇌학습기억능력；기궤제가능여기조절혈관성인지장애모형대서뇌내흥강성안기산(EAA)/억제성안기산(IAA)평형유관。
Objective To explore the influent of Bushe Huoxue decoction on neurotransmitter and its receptors in vascular cogni-tive impairment (VCI)in rats.Methods VCI models were established by bilateral common carotid arteries ligation.The contents of acetylcholine (ACh),serotonin (5 HT),dopamine (DA),glutamate (Glu),glycine (Gly),and gamma aminobutyric acid (GABA) in hippocampus were estimated with High Performance Liquid Chromatography (HPLC).The expression of the receptors of Ach,5 HT, DA,Glu,Gly and GABA were examined by immunohistochemistry.Results Bushe Huoxue decoction could increase the content of the Ach,5 HT,DA and GABA in hippocampus,and decrease the content of the Glu and Gly.Meanwhile,the expression of the receptors of Ach,5 HT,DA and GABA was increased,and the expression of the receptors of Glu and Gly was decreased.Conclusion Bushe Huoxue decoction might have some potential protective effects on neurons,and its possible mechanism was related to the balance of excitatory amino acids (EAA)and inhibitory amino acid (IAA).