CAJ | 학술논문

目的：建立一种快速、准确测定人血清中甲氨蝶呤浓度的高效液相色谱法。方法色谱柱为 Hypersil C18，流动相为甲醇-乙腈-磷酸盐缓冲液（2∶7∶91），流速为0.8 mL· min-1，检测波长313 nm；血清样品上SPE柱，以含甲醇-乙腈-磷酸盐缓冲液（50∶50∶250）为洗脱剂，收集洗脱液直接进样测定。结果甲氨蝶呤血清样品线性范围为0.03～50.00μg· mL-1，2组低、中、高不同浓度（A组：3.41，6.82，27.27μg· mL-1；B组：0.05，0.21，0.85μg· mL-1）方法回收率均大于97％。日内、日间RSD均小于5％。结论本方法准确性好、灵敏度高、操作简便，完全可满足儿科临床甲氨蝶呤血药浓度监测需要。
목적：건립일충쾌속、준학측정인혈청중갑안접령농도적고효액상색보법。방법색보주위 Hypersil C18，류동상위갑순-을정-린산염완충액（2∶7∶91），류속위0.8 mL· min-1，검측파장313 nm；혈청양품상SPE주，이함갑순-을정-린산염완충액（50∶50∶250）위세탈제，수집세탈액직접진양측정。결과갑안접령혈청양품선성범위위0.03～50.00μg· mL-1，2조저、중、고불동농도（A조：3.41，6.82，27.27μg· mL-1；B조：0.05，0.21，0.85μg· mL-1）방법회수솔균대우97％。일내、일간RSD균소우5％。결론본방법준학성호、령민도고、조작간편，완전가만족인과림상갑안접령혈약농도감측수요。
Objective To establish a rapid and accurate HPLC method for determination the concentration of methotrexate in human serum.Methods Chromatographic column was Hypersil C 18 column; mobile phase was methanol ,acetonitrile and phosphate buffer (2∶7∶91 ) , a flow rate was 0.8 mL· min-1;detection wavelength was 313 nm.Serum sam-ple was injected into SPE column.Methanol/acetonitrile/phosphate buff-er ( 50∶50∶250 ) was used as the eluent , and the collection of eluent was detected directly.Results The linear range of methotrexate in serum samples was 0.03-50.00 μg · mL-1 , coefficient of recoveries of low , medium and high concentrations in two groups ( group A:3.41 , 6.82 , 27.27 μg· mL-1;group B:0.05, 0.21, 0.85 μg· mL-1)were higher than 97%.Inter day and inner day RSD were less than 5%.Conclusion This method has good accuracy , high sensitivity , convenient opera-tion.It can fully satisfy the requirement of concentration monitoring of methotrexate in the pediatric clinical practice.