CAJ | 학술논문

目的：观察补骨脂素和异补骨脂素对HepG2细胞增殖、CYP基因表达及蛋白定位和蛋白表达的影响。方法用噻唑蓝（ MTT）比色法、流式细胞术、实时定量PCR、免疫荧光法（ IF）和 Western blot 检测补骨脂素和异补骨脂素对CYP1A1和CYP3A4的影响。结果在梯度浓度作用后，2种药物对HepG2细胞均有抑制作用，且存在明显的浓度依赖关系；2种药物（50μg · mL-1）均阻滞HepG2细胞周期于G2-M期，都是CYP1A1、CYP3A4 mRNA的抑制剂，但抑制效果有差别；CYP3A4蛋白表达均明显低于对照组。2种药物均抑制HepG2细胞增殖，主要是由于两者阻滞其周期于G2-M期。结论相比于补骨脂素，异补骨脂素对CYP3A4 mRNA表达和蛋白表达的抑制作用更强，而对CYP1A1mRNA的抑制强度略低。
목적：관찰보골지소화이보골지소대HepG2세포증식、CYP기인표체급단백정위화단백표체적영향。방법용새서람（ MTT）비색법、류식세포술、실시정량PCR、면역형광법（ IF）화 Western blot 검측보골지소화이보골지소대CYP1A1화CYP3A4적영향。결과재제도농도작용후，2충약물대HepG2세포균유억제작용，차존재명현적농도의뢰관계；2충약물（50μg · mL-1）균조체HepG2세포주기우G2-M기，도시CYP1A1、CYP3A4 mRNA적억제제，단억제효과유차별；CYP3A4단백표체균명현저우대조조。2충약물균억제HepG2세포증식，주요시유우량자조체기주기우G2-M기。결론상비우보골지소，이보골지소대CYP3A4 mRNA표체화단백표체적억제작용경강，이대CYP1A1mRNA적억제강도략저。
Objective To observe the effects of psoralen and isopsoralen on cell proliferation , CYP gene expression , protein localization and pro-tein expression of HepG2 cells.Methods The effects on CYP1A1 and CYP3 A4 of psoralen and isopsoralen were tested by MTT colorimetry , flow cytometry determination , real time quantitative PCR technology , im-mune -fluorescence and protein immune -blot method.Results HepG2 cells were inhibited at gradient concentration by psoralen and isopsoralen.What′s more , there was a clear dose -response relation-ship .The two drugs ( 50 μg · mL-1 ) blocked HepG2 cells cycle at G2-M phase .The two drugs were CYP1 A1 and CYP3 A4 mRNA inhibi-tors but with different effects .Protein expression was significantly lower than the control group.The proliferation of HepG2 cells are inhibited by the two drugs , because the cell cycle was arrested at G 2 -M phase.Conclusion Compared with psoralen , isopsoralen respectively inhibited more on mRNA and protein expression of CYP3A4, but the intensity of inhibition of CYP1 A1 mRNA was slightly lower.