CAJ | 학술논문

目的：观察成年大鼠脊髓损伤后内源性神经前体细胞的增殖与分化，探讨内源性神经前体细胞的自然变化规律。方法制作脊髓压迫损伤模型，BrdU 腹腔注射标记神经前体细胞，免疫荧光法（Immunofluorescence）检测大鼠脊髓 BrdU、GFAP、MBP 阳性细胞数的变化。结果1）正常组可观察到少量 BrdU 阳性细胞，脊髓损伤后 BrdU 阳性细胞显著增加（p ＜0.05），并在第7天达到最大值，21天时仍高水平表达。2）正常组可见少量 BrdU /GFAP 和 BrdU /MBP 阳性细胞，脊髓损伤后 BrdU /GFAP，BrdU /MBP 双标阳性细胞数显著增加（p ＜0.05）。结论脊髓损伤后神经前体细胞的数量在第7天达到最大值，我们认为，一周内可能是神经前体细胞增殖分化调控的关键时期。此外，新生星形胶质细胞和少突胶质细胞大量增殖，并与神经前体细胞的迁移、后肢功能恢复表现出一定的同步性，提示新生胶质细胞可能参与了脊髓损伤后神经功能的修复作用。
목적：관찰성년대서척수손상후내원성신경전체세포적증식여분화，탐토내원성신경전체세포적자연변화규률。방법제작척수압박손상모형，BrdU 복강주사표기신경전체세포，면역형광법（Immunofluorescence）검측대서척수 BrdU、GFAP、MBP 양성세포수적변화。결과1）정상조가관찰도소량 BrdU 양성세포，척수손상후 BrdU 양성세포현저증가（p ＜0.05），병재제7천체도최대치，21천시잉고수평표체。2）정상조가견소량 BrdU /GFAP 화 BrdU /MBP 양성세포，척수손상후 BrdU /GFAP，BrdU /MBP 쌍표양성세포수현저증가（p ＜0.05）。결론척수손상후신경전체세포적수량재제7천체도최대치，아문인위，일주내가능시신경전체세포증식분화조공적관건시기。차외，신생성형효질세포화소돌효질세포대량증식，병여신경전체세포적천이、후지공능회복표현출일정적동보성，제시신생효질세포가능삼여료척수손상후신경공능적수복작용。
Objective To investigate the proliferation and differentiation of the neural precursors and to explore the variation of neural precursors after spinal cord injury.Methods Spinal cord injury model (Allen's model)was used.BrdU was admin-istered intraperitoneally into rats,expression of BrdU,GFAP and MBP-positive cells were observed by immunofluorescence. Results (1)A small amount of BrdU positive cells were observed in control group,the positive cells were significantly in-creased after SCI,and reach the top in the 7th day group,and maintains a high level on the 21th day.(2)A small amount of BrdU /GFAP and BrdU /MBP double positive cells were observed in control group.There was a significant increase in double positive cells after SCI (p <0.05).Conclusion The neural precursor cells were significantly increased after SCI, which reached a maximum on the 7th day,we guess that within a week may be a critical period of neural precursor cell pro-liferation and differentiation.In addition,neonatal astrocytes and oligodendrocytes significantly increased after SCI,which meet the regular in migration of neural precursor cells and recovery of hindlimb functional,suggest that glial cells may be in-volved in neonatal repair of neurological function after spinal cord injury.