CAJ | 학술논문

为利用组合化学原理构建核酸适体库，确定久效磷适体筛选缓冲体系的最适 pH 值及离子强度，采用紫外吸收光谱法研究了不同 pH 值及不同 Na ﹢、K ﹢和 Mg2﹢浓度的缓冲溶液中久效磷与四种碱基的相互作用。结果表明，pH 值及 Na ﹢、K ﹢和 Mg2﹢浓度对久效磷与四种碱基的作用有明显的影响，pH7.6、Na ﹢浓度200 mmol/ L、K ﹢浓度40 mmol/ L、Mg2﹢浓度15 mmol/ L 的50 mmol/ L Tris 缓冲溶液中久效磷与四种碱基的作用相对较强，可以作为久效磷适体筛选的参考缓冲体系。在此缓冲体系下，久效磷与四种碱基作用的强弱依次为胞嘧啶、胸腺嘧啶、腺嘌呤、鸟嘌呤。因此，构建核酸适体库时可以适当增加胞嘧啶、胸腺嘧啶的比例。
위이용조합화학원리구건핵산괄체고，학정구효린괄체사선완충체계적최괄 pH 치급리자강도，채용자외흡수광보법연구료불동 pH 치급불동 Na ﹢、K ﹢화 Mg2﹢농도적완충용액중구효린여사충감기적상호작용。결과표명，pH 치급 Na ﹢、K ﹢화 Mg2﹢농도대구효린여사충감기적작용유명현적영향，pH7.6、Na ﹢농도200 mmol/ L、K ﹢농도40 mmol/ L、Mg2﹢농도15 mmol/ L 적50 mmol/ L Tris 완충용액중구효린여사충감기적작용상대교강，가이작위구효린괄체사선적삼고완충체계。재차완충체계하，구효린여사충감기작용적강약의차위포밀정、흉선밀정、선표령、조표령。인차，구건핵산괄체고시가이괄당증가포밀정、흉선밀정적비례。
In order to construct aptamer library using principles of combinatorial chemistry and determine pH and ionic strength of buffer when selecting monocrotophos aptamer,the interaction between monocrotophos and four bases including thymine,cytosine,adenine and guanine were studied by means of ultraviolet absorption spectrum in different pH values and concentration of Na﹢, K﹢ and Mg2﹢. The results showed that effect of pH values and concentration of Na﹢, K﹢ and Mg2﹢on interaction between monocrotophos and four bases was apparent,espe-cially at pH7.6, the concentration of 200 mmol/L Na﹢, 40mmol/L K﹢ and 15mmol/L Mg2﹢, the buffer could be used to select aptamer of monocrotophos. In the buffer system,the sequence of interaction between monocro-tophos and four bases was adenine,cytosine,thymine,guanine. So the ratio of cytosine and thymine can be in-creased when aptamer library will be constructed.