CAJ | 학술논문

半定量 RT-PCR 分析表明,OsGSTLc 在水稻根中的表达受绿磺隆的诱导.从水稻基因组中分离到的 OsGSTLc 读码框上游22171 bp 序列,在起始密码 ATG 上游-86 bp 处有 CAAT-box,但在 CAAT-box 与读码框之间没有典型的 TATA-box.因此,OsGSTLc 启动子是无 TATA 框启动子.将 OsGSTLc 启动子5′-端系列缺失后,分别与 GUS 报告基因融合,获得 GSTL2171：GUS 、GSTL 1761：GUS 、GSTL 962：GUS 和GSTL 525：GUS 表达载体,利用农杆菌介导转化水稻,获得转基因水稻,均能启动下游 GUS 报告基因的表达.氯磺隆处理后,转入 GSTL 2171：GUS 、GSTL 1761：GUS 和 GSTL 962：GUS 的水稻植株根部的 GUS活性明显增加.氯磺隆诱导的应答元件在-962~-525 bp 的范围内.
반정량 RT-PCR 분석표명,OsGSTLc 재수도근중적표체수록광륭적유도.종수도기인조중분리도적 OsGSTLc 독마광상유22171 bp 서렬,재기시밀마 ATG 상유-86 bp 처유 CAAT-box,단재 CAAT-box 여독마광지간몰유전형적 TATA-box.인차,OsGSTLc 계동자시무 TATA 광계동자.장 OsGSTLc 계동자5′-단계렬결실후,분별여 GUS 보고기인융합,획득 GSTL2171：GUS 、GSTL 1761：GUS 、GSTL 962：GUS 화GSTL 525：GUS 표체재체,이용농간균개도전화수도,획득전기인수도,균능계동하유 GUS 보고기인적표체.록광륭처리후,전입 GSTL 2171：GUS 、GSTL 1761：GUS 화 GSTL 962：GUS 적수도식주근부적 GUS활성명현증가.록광륭유도적응답원건재-962~-525 bp 적범위내.
The expression of OsGSTLc in rice roots was induced by chlorsulfuron through semi-quantitative RT-PCR analysis demonstrated.A 2 171 bp upstream sequence of the translation start codon ATG of OsGSTLc gene was iso-lated from the genomic DNA of rice,which contained a putative CAAT-box at position-86 bp upstream of ATG ,but there wasn’t TATA-box between the CAAT-box and ATG .Therefore,OsGSTL c promoter was a TATA-less pro-moter.To define the core promoter sequence,a series of 5′truncation derivatives of GST2171 were fused to a GUS reporter gene to construct GSTL 2171::GUS ,GSTL 1761::GUS ,GSTL 962::GUS and GSTL 525::GUS ,respec-tively.The four fusion genes were introduced into rice plants by Agrobacterium-mediated transformation,all promot-er fragments with 5′-deletion drived successfully the expression of GUS report gene.Quantitative fluorescence assays showed that the GUS activities in the roots of GSTL 2171::GUS ,GSTL 1761::GUS and GSTL 962::GUS transgen-ic rice seedlings were upregulated by chlorsulfuron.The transcriptional activation element of chlorsulfuron may be lo-cated between positions-962 and-525.