CAJ | 학술논문

鲨烯合酶(SQS )是植物甾醇和三萜化合物生物合成途径中的关键酶。以巴西橡胶树为试验材料,提取胶乳总 RNA,利用 RT-PCR 以及 RACE 的方法克隆橡胶树鲨烯合酶 cDNA 编码区片段,并进行序列分析。结果表明：橡胶树鲨烯合酶 cDNA 编码区为1239 bp,编码413个氨基酸,命名为 HbSQS 。荧光定量分析表明鲨烯合酶基因在不同组织里表达水平存在明显差异,且受乙烯调控。
사희합매(SQS )시식물치순화삼첩화합물생물합성도경중적관건매。이파서상효수위시험재료,제취효유총 RNA,이용 RT-PCR 이급 RACE 적방법극륭상효수사희합매 cDNA 편마구편단,병진행서렬분석。결과표명：상효수사희합매 cDNA 편마구위1239 bp,편마413개안기산,명명위 HbSQS 。형광정량분석표명사희합매기인재불동조직리표체수평존재명현차이,차수을희조공。
Squalene synthase (SQS )is a key enzyme in plant terpenoid biosynthetic pathway.The total RNA were extracted from Hevea brasiliensis relax,and the cDNA of squalene synthase was cloned using RT-PCR and RACE strategy.The results showed that the cDNA(named as HbSQS )contained a 1 239 bp open reading frame and encoded a predicted protein of 413 amino acids.SYBR Green I Real Time RT-PCR analysis indicated that the HbSQS was more highly expressed in bark than in leaf.The transcription of HbSQS in latex was induced by ethylene.