CAJ | 학술논문

以3年生圆柏和祁连圆柏幼苗为材料,采用不同浓度水杨酸(SA)预处理两种圆柏属植物幼苗,测定-4℃低温胁迫处理第6天叶片相对含水量(RWC)、相对电导率(REC)、丙二醛(MDA)、可溶性糖(SS)、可溶性蛋白(SP)、脯氨酸(Pro)和类胡萝卜素(Car)含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸氧化酶(APX)和谷胱甘肽还原酶(GR)活性,分析外源 SA 对低温胁迫下圆柏属植物叶片膜脂过氧化、渗透调节物质及抗氧化酶系统的影响,为培育较多品种的抗冷冻常绿植物提供理论依据。结果表明：低温胁迫下,一定浓度的 SA 预处理能有效保护幼苗叶片膜系统的稳定性、增加渗透调节物质含量和提高抗氧化酶活性,其中200 mg/L SA 预处理对提高圆柏抗寒性效果最好,30 mg/L SA 预处理对提高祁连圆柏抗寒性效果最好,且在外源 SA 预处理下,祁连圆柏的抗寒性比圆柏的强。因此,施用适合浓度的 SA 在提高圆柏属植物抗寒性方面具有较好的应用价值。
이3년생원백화기련원백유묘위재료,채용불동농도수양산(SA)예처리량충원백속식물유묘,측정-4℃저온협박처리제6천협편상대함수량(RWC)、상대전도솔(REC)、병이철(MDA)、가용성당(SS)、가용성단백(SP)、포안산(Pro)화류호라복소(Car)함량급초양화물기화매(SOD)、과양화물매(POD)、과양화경매(CAT)、항배혈산양화매(APX)화곡광감태환원매(GR)활성,분석외원 SA 대저온협박하원백속식물협편막지과양화、삼투조절물질급항양화매계통적영향,위배육교다품충적항냉동상록식물제공이론의거。결과표명：저온협박하,일정농도적 SA 예처리능유효보호유묘협편막계통적은정성、증가삼투조절물질함량화제고항양화매활성,기중200 mg/L SA 예처리대제고원백항한성효과최호,30 mg/L SA 예처리대제고기련원백항한성효과최호,차재외원 SA 예처리하,기련원백적항한성비원백적강。인차,시용괄합농도적 SA 재제고원백속식물항한성방면구유교호적응용개치。
The effect of exogenous SA on membrane lipid peroxidation,osmotic regulation substances and antioxidant enzyme activities in the leaves of Sabina species under low temperature stress at-4 ℃ for 6 d was analyzed by deter-mining the relative water content(RWC),relative electrical conductivity(REC),contents of malondialdehyde(MDA), soluble sugar(SS),soluble protein(SP),proline(Pro)and carotenoid(Car),activities of superoxide dismutase(SOD), peroxidase(POD),catalase(CAT),ascorbic acid peroxidase(APX)and glutathione reductase(GR)in the leaves of 3-year-old seedlings of S .przewalskii and S .chinensis pretreated with different concentrations of SA to provide a theo-retical basis for fostering more anti-freeze evergreen varieties.The results indicated that certain concentrations of SA could maintain the stability of membrane system and increase the contents of osmotic regulators and antioxidant en-zyme activities,enhance freezing tolerance of plant,the optimum SA concentration was found to be 200 mg/L for S . przewalskii and 30 mg/L for S .chinensis .The chilling tolerance of S .chinensis was stronger than that of S .przew-alskii in the pretreatments with SA.Those results suggested that appropriate concentration of SA could help to in-crease the chilling resistance of Sabina species.