CAJ | 학술논문

目的：探讨急性白血病流式细胞术免疫分型的意义以及与FAB分型的对照。方法采用流式细胞术三色荧光标记技术和CD45/SC双参数散点图设门，检测175例急性白血病的免疫表型，对抗原表达情况进行分析。结果 FAB分型和免疫分型的相符率90.28%（158/175），175例急性白血病检出B-ALL44例，占25.14%，其主要表达的抗原阳性率从高到低依次为CD19、CD22、CD79a；T-ALL19例，占10.86%，其主要表达的抗原阳性率从高到低依次为HLA-DR、CD34、CD5、CD7、cCD3、CD8、CD38；非M3 AML103例，占58.86%，其主要表达的抗原阳性率从高到低依次为CD33、CD117、CD38、CD13、HLA-DR、MPO、CD34、CD123、CD15、CD64；共检出AML-M3患者6例，占0.34%，其主要表达的抗原阳性为MPO、CD13、CD33、CD117、CD123、CD64。结论流式细胞术免疫分型在白血病分型中起重要作用，是FAB分型的补充和修正,提高了急性白血病诊断的准确率，有必要进一步加强流式细胞术免疫分型的规范化和标准化工作。
목적：탐토급성백혈병류식세포술면역분형적의의이급여FAB분형적대조。방법채용류식세포술삼색형광표기기술화CD45/SC쌍삼수산점도설문，검측175례급성백혈병적면역표형，대항원표체정황진행분석。결과 FAB분형화면역분형적상부솔90.28%（158/175），175례급성백혈병검출B-ALL44례，점25.14%，기주요표체적항원양성솔종고도저의차위CD19、CD22、CD79a；T-ALL19례，점10.86%，기주요표체적항원양성솔종고도저의차위HLA-DR、CD34、CD5、CD7、cCD3、CD8、CD38；비M3 AML103례，점58.86%，기주요표체적항원양성솔종고도저의차위CD33、CD117、CD38、CD13、HLA-DR、MPO、CD34、CD123、CD15、CD64；공검출AML-M3환자6례，점0.34%，기주요표체적항원양성위MPO、CD13、CD33、CD117、CD123、CD64。결론류식세포술면역분형재백혈병분형중기중요작용，시FAB분형적보충화수정,제고료급성백혈병진단적준학솔，유필요진일보가강류식세포술면역분형적규범화화표준화공작。
Obiective To investigate the significance of immunophenotype in acute leukemia via flow cytometry, and study the relationship of immunophenotype and FAB phenotype. Methods Immunophenotypes were detected by three-color flow cytometry(FCM )and CD45/SSC gating in 175 cases of acute leukemia(AL)and antigentic expressions were analysed. Results FAB classification and immunophenotype consistent rate of 90.28%(158/175), 175 cases of acute leukemia detected in B-ALL44 cases, accounting for 25.14%, antigen positive rate of its main expression in the order of CD19, CD22, CD79a;T-ALL19 cases, accounting for 10.86%, the positive rate of antigen expression and its main descending order of HLA-DR, CD34, CD5, CD7, cCD3, CD8, CD38;non M3 AML103 cases, accounting for 58.86%, its the positive rate of antigen expression in the order of major CD33, CD117, CD38, CD13, HLA-DR, MPO, CD34, CD123, CD15, CD64;were found in AML-M3 6 patients, accounting for 0.34%, and its main antigen expression of MPO, CD13, CD33, CD117, CD123, CD64. Conclusion Immunophenotype Plays an important role in the classification of acute leukemia and is a supplementary and correction of FAB phenotype. It can enhance the exactness of diagnosis and needs to be standardized.