中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2014年
31期
3-4
,共2页
白细胞介素-33%慢性阻塞性肺病%实时荧光定量RT-PCR
白細胞介素-33%慢性阻塞性肺病%實時熒光定量RT-PCR
백세포개소-33%만성조새성폐병%실시형광정량RT-PCR
Interleukin-33%Chronic obstructive pulmonary disease%Real-time fluorescent quantitative RT-PCR
目的：研究白细胞介素-33（IL-33）在慢性阻塞性肺疾病（COPD）患者体内的表达及其因病情不同转归而动态改变的规律。方法31例COPD急性加重期（AECOPD）患者依据GOLD诊断分级标准分三组：轻度组，中度组和重-极重度组，实时荧光定量RT-PCR检测患者治疗前后外周血单个核细胞IL-33的基因表达。结果健康人及轻度COPD患者IL-33基因表达维持在低水平，中度和重-极重度患者其表达明显增加，急性加重时升高尤为明显，治疗后病情缓解IL-33表达水平也随之下调。结论 COPD患者体内表达IL-33水平随病情严重程度而升高，病情恶化或好转时，IL-33表达水平都随之同步改变。
目的：研究白細胞介素-33（IL-33）在慢性阻塞性肺疾病（COPD）患者體內的錶達及其因病情不同轉歸而動態改變的規律。方法31例COPD急性加重期（AECOPD）患者依據GOLD診斷分級標準分三組：輕度組，中度組和重-極重度組，實時熒光定量RT-PCR檢測患者治療前後外週血單箇覈細胞IL-33的基因錶達。結果健康人及輕度COPD患者IL-33基因錶達維持在低水平，中度和重-極重度患者其錶達明顯增加，急性加重時升高尤為明顯，治療後病情緩解IL-33錶達水平也隨之下調。結論 COPD患者體內錶達IL-33水平隨病情嚴重程度而升高，病情噁化或好轉時，IL-33錶達水平都隨之同步改變。
목적：연구백세포개소-33（IL-33）재만성조새성폐질병（COPD）환자체내적표체급기인병정불동전귀이동태개변적규률。방법31례COPD급성가중기（AECOPD）환자의거GOLD진단분급표준분삼조：경도조，중도조화중-겁중도조，실시형광정량RT-PCR검측환자치료전후외주혈단개핵세포IL-33적기인표체。결과건강인급경도COPD환자IL-33기인표체유지재저수평，중도화중-겁중도환자기표체명현증가，급성가중시승고우위명현，치료후병정완해IL-33표체수평야수지하조。결론 COPD환자체내표체IL-33수평수병정엄중정도이승고，병정악화혹호전시，IL-33표체수평도수지동보개변。
Objective To study the expression of Interleukin-33(IL-33) in patients with chronic obstructive pulmonary disease (COPD) and its dynamic changes with different respond to treatment. Methods 31 patients with acute exacerbation chronic obstructive pulmonary disease (AECOPD) were grouped according to the GOLD COPD classifications:mild group, moderate group and severe-very severe group. The expression of IL-33 mRNA in peripheral blood mononuclear cells was determined by Real-time fluorescent quantitative RT-PCR before and after treatment. Results The expression of IL-33 mRNA was maintained at a low level in control group and mild group, but significantly increased in moderate group and severe-very severe group. The upward trend was especially apparent in COPD patients at acute exacerbation phase. When condition improved, the level was downwad. Conclusion The expression of IL-33 in patients with COPD is increased with the severity of the disease and changed simultaneously when condition deteriorated or improved.
