天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
11期
1132-1134
,共3页
心绞痛,不稳定型%白细胞介素18%腺苷%受体,腺苷A1%受体,腺苷A2%受体,腺苷A3
心絞痛,不穩定型%白細胞介素18%腺苷%受體,腺苷A1%受體,腺苷A2%受體,腺苷A3
심교통,불은정형%백세포개소18%선감%수체,선감A1%수체,선감A2%수체,선감A3
angina,unstable%interleukin-18%adenosine%receptor,adenosine A1%receptor,adenosine A2%receptor,adenosine A3
目的：观察腺苷及腺苷受体激动剂对不稳定型心绞痛（UAP）患者白介素（IL）-18的影响，探讨腺苷受体的作用机制。方法选取15例UAP患者（UAP组）及15例健康志愿者（对照组），采用腺苷及腺苷受体激动剂干预全血标本，双抗体夹心酶联免疫吸附法（ELISA）检测IL-18。结果低浓度腺苷(1～100μmol/L)对UAP组IL-18表达有增强效应，高浓度(1 mmol/L)有抑制效应。腺苷A1受体激动剂与A3受体激动剂对IL-18表达有增强效应，而腺苷A2a受体激动剂对IL-18表达有抑制效应，腺苷A2b受体激动剂对IL-18表达未见明显效应。结论腺苷在低浓度时对UAP患者IL-18的表达有促进作用，可能通过腺苷A1和A3受体发挥效应；高浓度时有抑制作用，可能通过腺苷A2a受体发挥作用。
目的：觀察腺苷及腺苷受體激動劑對不穩定型心絞痛（UAP）患者白介素（IL）-18的影響，探討腺苷受體的作用機製。方法選取15例UAP患者（UAP組）及15例健康誌願者（對照組），採用腺苷及腺苷受體激動劑榦預全血標本，雙抗體夾心酶聯免疫吸附法（ELISA）檢測IL-18。結果低濃度腺苷(1～100μmol/L)對UAP組IL-18錶達有增彊效應，高濃度(1 mmol/L)有抑製效應。腺苷A1受體激動劑與A3受體激動劑對IL-18錶達有增彊效應，而腺苷A2a受體激動劑對IL-18錶達有抑製效應，腺苷A2b受體激動劑對IL-18錶達未見明顯效應。結論腺苷在低濃度時對UAP患者IL-18的錶達有促進作用，可能通過腺苷A1和A3受體髮揮效應；高濃度時有抑製作用，可能通過腺苷A2a受體髮揮作用。
목적：관찰선감급선감수체격동제대불은정형심교통（UAP）환자백개소（IL）-18적영향，탐토선감수체적작용궤제。방법선취15례UAP환자（UAP조）급15례건강지원자（대조조），채용선감급선감수체격동제간예전혈표본，쌍항체협심매련면역흡부법（ELISA）검측IL-18。결과저농도선감(1～100μmol/L)대UAP조IL-18표체유증강효응，고농도(1 mmol/L)유억제효응。선감A1수체격동제여A3수체격동제대IL-18표체유증강효응，이선감A2a수체격동제대IL-18표체유억제효응，선감A2b수체격동제대IL-18표체미견명현효응。결론선감재저농도시대UAP환자IL-18적표체유촉진작용，가능통과선감A1화A3수체발휘효응；고농도시유억제작용，가능통과선감A2a수체발휘작용。
Objective To observe the effects of adenosine and adenosine receptor agonist on the expression of inter?leukin-18 (IL-18) in patients with unstable angina (UAP), and the mechanism of adenosine receptor agonists thereof. Meth?ods Fifteen UAP and 15 healthy volunteers were included in this study. The effects of adenosine and the selective adenos?ine receptor agonists on the expression of IL-18 were measured by enzyme-linked immunosorbent assay (ELISA). Results The lower concentration of adenosine (1-100μmol/L) increased the expression of IL-18 in UAP group;whereas the higher concentration of adenosine (1 mmol/L) inhibited the expression of IL-18. The adenosine A1 receptor agonist and A3 receptor agonist increased the expression of IL-18, while the adenosine A2a receptor agonist inhibited the expression of IL-18. There was no significant effect for A2b receptor agonist on the expression of IL-18. Conclusion The lower concentrations of ade?nosine can enhance the expression of IL-18 through adenosine A1 and A3 receptors in UAP. The higher concentrations of ade?nosine can inhibit the expression of IL-18 through adenosine A2a receptors. Adenosine can promote IL-18 expression in lower concentrations.
