CAJ | 학술논문

目的：探讨黄芪多糖诱导成熟的树突状细胞（DC）肿瘤疫苗对S180荷瘤小鼠的抗肿瘤作用及作用机制。方法体外培养小鼠骨髓来源的DC，加入黄芪多糖诱导成熟，以S180肿瘤抗原致敏获得DC肿瘤疫苗。建立S180荷瘤小鼠模型，分为模型组、环磷酰胺组、黄芪多糖组、细胞因子组，在荷瘤第5天和第10天分别给予相应治疗。荷瘤第12天摘取肿瘤组织、胸腺、脾脏称质量，计算抑瘤率、胸腺指数、脾脏指数；ELISA法检测小鼠血清白细胞介素（IL）-4、干扰素（IFN）-γ水平。结果黄芪多糖组、细胞因子组的抑瘤率高于环磷酰胺组（64.25%、64.10%vs 35.11%），胸腺指数高于环磷酰胺组（1.69±0.26、1.74±0.38 vs 1.45±0.22），脾脏指数高于环磷酰胺组(5.44±0.76、5.31±0.81 vs 3.54±0.52)，IL-4水平(ng/L)低于环磷酰胺组（15.66±2.57、14.72±4.84 vs 23.95±6.07），IFN-γ水平(ng/L)高于环磷酰胺组（16.54±3.71、17.20±2.03 vs 10.37±2.19），差异均有统计学意义。结论黄芪多糖诱导的DC疫苗可有效发挥抑瘤作用，其机制可能与提高荷瘤小鼠胸腺指数与脾脏指数，调节细胞因子表达，促进Th1/Th2失衡向Th1细胞占优势的细胞免疫偏移，增强机体的抗肿瘤免疫功能有关。
목적：탐토황기다당유도성숙적수돌상세포（DC）종류역묘대S180하류소서적항종류작용급작용궤제。방법체외배양소서골수래원적DC，가입황기다당유도성숙，이S180종류항원치민획득DC종류역묘。건립S180하류소서모형，분위모형조、배린선알조、황기다당조、세포인자조，재하류제5천화제10천분별급여상응치료。하류제12천적취종류조직、흉선、비장칭질량，계산억류솔、흉선지수、비장지수；ELISA법검측소서혈청백세포개소（IL）-4、간우소（IFN）-γ수평。결과황기다당조、세포인자조적억류솔고우배린선알조（64.25%、64.10%vs 35.11%），흉선지수고우배린선알조（1.69±0.26、1.74±0.38 vs 1.45±0.22），비장지수고우배린선알조(5.44±0.76、5.31±0.81 vs 3.54±0.52)，IL-4수평(ng/L)저우배린선알조（15.66±2.57、14.72±4.84 vs 23.95±6.07），IFN-γ수평(ng/L)고우배린선알조（16.54±3.71、17.20±2.03 vs 10.37±2.19），차이균유통계학의의。결론황기다당유도적DC역묘가유효발휘억류작용，기궤제가능여제고하류소서흉선지수여비장지수，조절세포인자표체，촉진Th1/Th2실형향Th1세포점우세적세포면역편이，증강궤체적항종류면역공능유관。
Objective To study the antitumor effects of dendritic cell vaccine induced by astragalus polysaccha?rides on S180 tumor-bearing mice, and its possible mechanism. Methods Dendritic cells derived from mouse bone marrow were induced maturation by astragalus polysaccharides and loaded with S180 tumor antigen to prepare tumor vaccine. Tu?mor-bearing mice were divided into four groups and treated on day-5 and day-10 respectively. Group A was injected with NS, Group B with CTX (50 mg/kg), Group C with dendritic cells induced by astragalus polysaccharides and Group D with dendritic cells induced by tumor necrosis factor (TNF)-α. After 12 days of tumor-bearing, the animals were killed. The sub?cutaneous sarcoma, thymus and spleen were separated and weighted. The inhibitory rate, thymus index and spleen index were then calculated. ELISA assay was used to detect the levels of interleukin (IL)-4, interferon (IFN)-γin serum of tumor bearing mice. Results The tumor inhibition rate was higher in astragalus polysaccharide group and cytokine group than that of CTX group (64.25%, 64.10%vs 35.11%). The thymus index was higher in astragalus polysaccharide group and cyto?kine group than that of CTX group (1.69 ± 0.26, 1.74 ± 0.38 vs 1.45 ± 0.22). The spleen index was higher in astragalus poly?saccharide group and cytokine group than that of CTX group (5.44 ± 0.76, 5.31 ± 0.81 vs 3.54 ± 0.52). The level of IL-4 was lower in astragalus polysaccharide group and cytokine group than that of CTX group (15.66±2.57, 14.72 ± 4.84 vs 23.95 ± 6.07). The level of IFN-γwas higher in astragalus polysaccharide group and cytokine group than that of CTX group (16.54 ± 3.71, 17.20 ± 2.03 vs 10.37 ± 2.19). All the differences were statistically significant (P<0.05). Conclusion Dendritic cell vaccine induced by astragalus polysaccharides can effectively inhibit tumor growth. Its mechanism may be associated with the promotion spleen index and thymus index of S180 tumor-bearing mice, the effective correction of Th1/Th2 imbalance in?duced by tumor, and the enhancement of antitumor immune responses.