CAJ | 학술논문

目的：探讨碳青霉烯类耐药的克雷伯菌属细菌的耐药机制，了解获得性碳青霉烯类耐药克雷伯菌属细菌流行状况，建立克雷伯菌属细菌的脉冲场凝胶电泳（PFGE）分型数据库，以便在发生医院暴发流行时快速溯源，为控制该类细菌的传播提供技术支撑。方法收集碳青霉烯类耐药的克雷伯菌属细菌，用改良 Hodge 试验和聚合酶链反应（PCR）分别测定其表现型和基因型；用 PFGE 对其进行同源性分析。结果碳青霉烯类耐药的克雷伯菌属细菌的耐药机制以产碳青霉烯酶（KPC）为主，PFGE 分析的8株肺炎克雷伯菌可分成 A、B 两种PFGE 型，其中 A 型4株、B 型4株。结论产 KPC 是本院克雷伯菌属细菌耐碳青霉烯类抗菌药物的主要原因，应重视医院细菌耐药性监测及医院感染的监控。
목적：탐토탄청매희류내약적극뢰백균속세균적내약궤제，료해획득성탄청매희류내약극뢰백균속세균류행상황，건립극뢰백균속세균적맥충장응효전영（PFGE）분형수거고，이편재발생의원폭발류행시쾌속소원，위공제해류세균적전파제공기술지탱。방법수집탄청매희류내약적극뢰백균속세균，용개량 Hodge 시험화취합매련반응（PCR）분별측정기표현형화기인형；용 PFGE 대기진행동원성분석。결과탄청매희류내약적극뢰백균속세균적내약궤제이산탄청매희매（KPC）위주，PFGE 분석적8주폐염극뢰백균가분성 A、B 량충PFGE 형，기중 A 형4주、B 형4주。결론산 KPC 시본원극뢰백균속세균내탄청매희류항균약물적주요원인，응중시의원세균내약성감측급의원감염적감공。
Objective To investigate the resistance mechanism and epidemiology of carbapenem-resistant Klebsiella spp.,and to establish the pulsed-field gel electrophoresis(PFGE)typing database of carbapenem-resistant Klebsiella spp.,in order to provide the reference for rapidly source-tracking and controlling the bacterium spread at the outbreak of nosocomial infection.Methods Carbapenem-resistant Klebsiella spp.were collected,and the phenotypic and genetic features were detected by modified Hodge test and polymerase chain reaction(PCR),respectively.PFGE typing was performed for homology analysis.Results The main resistance mechanism of carbapenem-resistant Klebsiella spp.was Klebsiella pneumoniae carbapenemase (KPC )-producing,and there were 2 genotypes by PFGE typing,including genotype A(4 isolates)and genotype B(4 isolates).Conclusions KPC-producing is the main reason for carbapenem-resistant Klebsiella spp.in the hospital,and it is necessary to monitor the resistance and to strengthen the infection control.