现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
19期
134-136
,共3页
辣椒%土壤%苯醚甲环唑%气相色谱%残留分析
辣椒%土壤%苯醚甲環唑%氣相色譜%殘留分析
랄초%토양%분미갑배서%기상색보%잔류분석
pepper%soil%difenoconazole%gas chromatography%determination analysis
建立了辣椒和土壤样品中苯醚甲环唑残留量的快速检测方法，样品于分液漏斗中用正己烷萃取，振荡提取3次，浓缩后正己烷定容，气相色谱-电子捕获检测器(GC-ECD)检测，外标法定量。结果表明：正己烷提取辣椒和土壤中苯醚甲环唑效果较好，无需净化可以直接上机检测。该方法在苯醚甲环唑添加量为0.01~1.00 mg/L范围内线性相关性良好，R2=0.9971，最低检出限为0.0025 mg/kg。在添加水平为0.01~1.00 mg/kg中，辣椒在3个添加水平下的苯醚甲环唑回收率为83.8%~102.2%，相对标准偏差为3.9%~4.9%；土壤在3个添加水平下的苯醚甲环唑回收率为83.7%~104.4%，相对标准偏差为6.7%~8.4%。它具有高效率、低成本、高灵敏度、定量准确等优点。
建立瞭辣椒和土壤樣品中苯醚甲環唑殘留量的快速檢測方法，樣品于分液漏鬥中用正己烷萃取，振盪提取3次，濃縮後正己烷定容，氣相色譜-電子捕穫檢測器(GC-ECD)檢測，外標法定量。結果錶明：正己烷提取辣椒和土壤中苯醚甲環唑效果較好，無需淨化可以直接上機檢測。該方法在苯醚甲環唑添加量為0.01~1.00 mg/L範圍內線性相關性良好，R2=0.9971，最低檢齣限為0.0025 mg/kg。在添加水平為0.01~1.00 mg/kg中，辣椒在3箇添加水平下的苯醚甲環唑迴收率為83.8%~102.2%，相對標準偏差為3.9%~4.9%；土壤在3箇添加水平下的苯醚甲環唑迴收率為83.7%~104.4%，相對標準偏差為6.7%~8.4%。它具有高效率、低成本、高靈敏度、定量準確等優點。
건립료랄초화토양양품중분미갑배서잔류량적쾌속검측방법，양품우분액루두중용정기완췌취，진탕제취3차，농축후정기완정용，기상색보-전자포획검측기(GC-ECD)검측，외표법정량。결과표명：정기완제취랄초화토양중분미갑배서효과교호，무수정화가이직접상궤검측。해방법재분미갑배서첨가량위0.01~1.00 mg/L범위내선성상관성량호，R2=0.9971，최저검출한위0.0025 mg/kg。재첨가수평위0.01~1.00 mg/kg중，랄초재3개첨가수평하적분미갑배서회수솔위83.8%~102.2%，상대표준편차위3.9%~4.9%；토양재3개첨가수평하적분미갑배서회수솔위83.7%~104.4%，상대표준편차위6.7%~8.4%。타구유고효솔、저성본、고령민도、정량준학등우점。
A rapid analytical method was developed for the determination of difenoconazole residue in pepper by gas chromatography(GC).The difenoconazole was extracted by n-hexane in a separatory funnel,and oscillation and extracting for 3 times,then determined by GC-ECD with external standard method.The results showed that n-hexane used as extract solvent was favorable for the extraction of difenoconazole without purification.A good linearity ranged from 0.01~1.00 mg/L was obtained and the correlation coefficient was 0.997 1.The limit of detection(LOD)was 0.002 5 mg/kg.The average recovery of difenoeonazole in pepper and soil was 83.8%~102.2%and 83.7%~104.4%,respectively at three spiked levels.The corresponding relation standard deviation(RSD)was 3.9%~4.9%and 6.7%~8.4%,respectively.Compared with the other methods,this method has the advantages of high efficiency,low cost,high sensitivity,accurate etc..
