CAJ | 학술논문

目的：应用快速液相色谱-高分辨飞行时间质谱（ RRLC-TOFMS）技术对中药艾叶中化学成分进行快速鉴别。方法色谱分离采用Agilent Eclipse C18色谱柱（2．1 mm ×100 mm，1．8μm）；流动相：乙腈（A）-0．1％甲酸水溶液（B），梯度洗脱，A相随时间的变化：5％～30％（0～12 min），30％～80％（12～25 min），80％（25～30 min），进样量1μl，流速0．35 ml／min，柱温40℃。 TOFMS、电喷雾离子源（ ESI）、正离子模式、质量数扫描范围m／z 100～1500。结果一次性鉴别出艾叶中31种化学成分。结论建立基于RRLC-TOFMS技术对艾叶中化学成分快速鉴别的方法，为中药艾叶的质量控制及体内的深入研究奠定基础。
목적：응용쾌속액상색보-고분변비행시간질보（ RRLC-TOFMS）기술대중약애협중화학성분진행쾌속감별。방법색보분리채용Agilent Eclipse C18색보주（2．1 mm ×100 mm，1．8μm）；류동상：을정（A）-0．1％갑산수용액（B），제도세탈，A상수시간적변화：5％～30％（0～12 min），30％～80％（12～25 min），80％（25～30 min），진양량1μl，류속0．35 ml／min，주온40℃。 TOFMS、전분무리자원（ ESI）、정리자모식、질량수소묘범위m／z 100～1500。결과일차성감별출애협중31충화학성분。결론건립기우RRLC-TOFMS기술대애협중화학성분쾌속감별적방법，위중약애협적질량공제급체내적심입연구전정기출。
Objective To identify the chemical components in Artemisiae argyi folium by rapid resolution liquid chromatog-raphy-time of flight mass spectrometry (RRLC-TOFMS).Methods The separation was performed on an Agilent Eclipse C18 column (2.1 mm ×100 mm,1.8 μm ).The mobile phase consisted of acetonitrile (A) and 0.1% formic acid (B) were in gradient elu-tion.The flowing rate was 0.35 ml/min, the injection volume was 1μl and the temperature of column was 40℃.Time of flight mass spectrometer ( TOFMS) with electro spray ion source ( ESI) was applied to qualitative analysis under the positive ion mode, and mass scan range was m/z 100-1 500 .Results 31 chemical compounds in Artemisiae argyi folium were identified unequivocally .Conclu-sion A rapid and efficient RRLC-TOFMS approach for identifying the chemical constituents of Artemisiae argyi folium had been suc-cessfully established,which paved a way for quality control and further in vivo studies of Artemisiae argyi folium.