CAJ | 학술논문

目的：：探讨姜黄素对Jurkat细胞增殖及凋亡的影响。方法：体外培养条件下,用姜黄素处理Jurkat细胞12h后,MTT法检测细胞的生存率和IC50值；Hoechst33342染色观察细胞凋亡的形态变化；流式细胞学检测细胞凋亡率。结果：体外培养条件下,姜黄素对Jurkat细胞的增殖有明显抑制作用,呈剂量依赖性,IC50值为31μmol/L。激光共聚焦扫描显微镜观察,随着姜黄素浓度的增加,Jurkat细胞凋亡增多。流式细胞学分析表明,低浓度姜黄素使Jurkat细胞发生早期凋亡,高浓度姜黄素使Jurkat细胞出现晚期凋亡。结论：姜黄素明显抑制Jurkat细胞增殖,同时诱导Jurkat细胞发生凋亡。
목적：：탐토강황소대Jurkat세포증식급조망적영향。방법：체외배양조건하,용강황소처리Jurkat세포12h후,MTT법검측세포적생존솔화IC50치；Hoechst33342염색관찰세포조망적형태변화；류식세포학검측세포조망솔。결과：체외배양조건하,강황소대Jurkat세포적증식유명현억제작용,정제량의뢰성,IC50치위31μmol/L。격광공취초소묘현미경관찰,수착강황소농도적증가,Jurkat세포조망증다。류식세포학분석표명,저농도강황소사Jurkat세포발생조기조망,고농도강황소사Jurkat세포출현만기조망。결론：강황소명현억제Jurkat세포증식,동시유도Jurkat세포발생조망。
Objective:To investigate effect of curcumin on proliferation and apoptosis of Jurkat cells. Methods:Under in vitro conditions, Jurkat cells were treated with different concentrations of curcumin for 12h. The cells were harvested for MTT analysis (sur-vival rate and IC50). Meanwhile, the apoptosis cells were determined with Hoechst33342 staining for morphologic changes and flow cytometry for apoptotic rate. Results: The proliferation of Jurkat cells was obviously inhibited in a dose-dependent manner ( IC50=31μmol/L) by curcumin under the in vitro conditions. The number of apoptosis cells increased with the concentration of curcumin un-der confocal laser microscope. After analyzed with flow cytometry, it found that early apoptosis cells were more in lower curcumin con-centration, and late apoptosis cells were much more in higher curcumin concentration. Conclusions:Curcumin inhibits the proliferation of Jurkat cells and induces apoptosis of Jurkat cells.