CAJ | 학술논문

根据已克隆的田旋花（ Convolvulus arvensis L．） EPSPS基因mRNA序列设计引物，通过染色体步移技术获得了1142 bp的EPSPS-P启动子片段（ GenBank 登录号：KC107822）。对启动子序列分析显示，该片段富含A/T碱基、TATA-box、CAAT-box及其他作用元件如GATA-motif、TC-rich repeats、sp1等。将该启动子与GUS报告基因连接以构建植物表达载体，利用农杆菌介导法获得转基因拟南芥；PCR和GUS组织化学染色分析证明， EPSPS-P已转化到拟南芥中。
근거이극륭적전선화（ Convolvulus arvensis L．） EPSPS기인mRNA서렬설계인물，통과염색체보이기술획득료1142 bp적EPSPS-P계동자편단（ GenBank 등록호：KC107822）。대계동자서렬분석현시，해편단부함A/T감기、TATA-box、CAAT-box급기타작용원건여GATA-motif、TC-rich repeats、sp1등。장해계동자여GUS보고기인련접이구건식물표체재체，이용농간균개도법획득전기인의남개；PCR화GUS조직화학염색분석증명， EPSPS-P이전화도의남개중。
Primers were designed according to the cloned EPSPS gene mRNA of Convolvulus arvensis L.A 1 142 bp pro-moter sequence named EPSPS-P ( Genbank accession number:KC107822 ) was obtained by genome walking technolo-gy.Sequence analysis of the promoter indicate that the fragment is rich in A/T base,TATA-box,CAAT-box and other acting elements such as GATA-motifs,TC-rich repeats,and sp1.Connecting this promoter with GUS report gene al-lowed construction of a plant expression vector.Agrobacterium tumefaciens-mediated transformation was used to trans-form Arabidopsis thaliana.Analysis of PCR results and GUS histochemical dyeing proved that EPSPS-P was introduced into A.thaliana.