CAJ | 학술논문

目的：建立高效液相色谱（high efficiency liquid chromatography，HPLC）法测定交沙霉素原料药中主组分的含量。方法采用十八烷基键合硅胶柱（C18）为固定相，以高氯酸溶液（取高氯酸钠-水合物119g，加水至1000 mL，用1mol/L的盐酸调节 pH 值为2．5）-乙腈（60∶40）为流动相。柱温为40℃，检测波长为231nm，进样量为20μL。结果交沙霉素主组分在考察的浓度范围内线性关系良好，线性范围为0．1255～0．8785g/L（r ＝1．0000），平均回收率为99．7％。定量限为2．51ng，最低检出限为0．84ng。结论 HPLC 可用于交沙霉素原料药中主组分含量的测定，快速简便，准确可靠，灵敏度高。
목적：건립고효액상색보（high efficiency liquid chromatography，HPLC）법측정교사매소원료약중주조분적함량。방법채용십팔완기건합규효주（C18）위고정상，이고록산용액（취고록산납-수합물119g，가수지1000 mL，용1mol/L적염산조절 pH 치위2．5）-을정（60∶40）위류동상。주온위40℃，검측파장위231nm，진양량위20μL。결과교사매소주조분재고찰적농도범위내선성관계량호，선성범위위0．1255～0．8785g/L（r ＝1．0000），평균회수솔위99．7％。정량한위2．51ng，최저검출한위0．84ng。결론 HPLC 가용우교사매소원료약중주조분함량적측정，쾌속간편，준학가고，령민도고。
Objective A high efficiency liquid chromatography method was established for the determination of principal component crude drug josamycin.Methods A octadecyl bonded silica column was used as the stationary phase,with the mobile phase of mixture of perchloric acid solution-acetonitrile (60∶40)at the detection wavelength of 231nm.The column temperature was 40℃ and the injection volume was 20μL.Results The principal component of josamycin showed a linear range within 0.125 5- 0.878 5g/L (r = 1.000 0).The average recovery was 99.7%.The quantitation and detection limits were 2.51ng and 0.84ng,respectively.Conclusion The method was rapid,simple,accurate,sensitive,and suitable for the determination of principal component in crude drug josamycin.