CAJ | 학술논문

目的：探讨美金胺对大鼠全脑缺血/再灌注（global cerebral ischemia/reperfusion，I/R）诱导学习记忆障碍的影响。方法通过4动脉结扎建立大鼠全脑缺血模型，在缺血后连续5 d腹腔注射美金胺，采用 Morris水迷宫进行行为学测定，通过焦油紫染色观察海马神经元的存活情况。结果与生理盐水对照组比较，假手术组和美金胺组逃逸潜伏期明显缩短（P＜0．01）；I/R组逃逸潜伏期明显长于假手术组和美金胺组（P＜0．01），与生理盐水对照组差异无统计学意义（P＞0．05）；与生理盐水对照组比较，假手术组和美金胺组穿越平台时间、在原西北象限游泳的百分比和海马CA1区神经细胞数明显增多（P＜0．01），而 I/R组穿越平台时间、在原象限游泳的百分比和海马区神经细胞较假手术组和美金胺组明显减少（P＜0．01），但仍与生理盐水对照组比较差异无统计学意义（P＞0．05）。结论美金胺可以改善大鼠全脑缺血再灌注所致的学习记忆障碍，该作用可能通过减轻缺血性神经元损伤实现。
목적：탐토미금알대대서전뇌결혈/재관주（global cerebral ischemia/reperfusion，I/R）유도학습기억장애적영향。방법통과4동맥결찰건립대서전뇌결혈모형，재결혈후련속5 d복강주사미금알，채용 Morris수미궁진행행위학측정，통과초유자염색관찰해마신경원적존활정황。결과여생리염수대조조비교，가수술조화미금알조도일잠복기명현축단（P＜0．01）；I/R조도일잠복기명현장우가수술조화미금알조（P＜0．01），여생리염수대조조차이무통계학의의（P＞0．05）；여생리염수대조조비교，가수술조화미금알조천월평태시간、재원서북상한유영적백분비화해마CA1구신경세포수명현증다（P＜0．01），이 I/R조천월평태시간、재원상한유영적백분비화해마구신경세포교가수술조화미금알조명현감소（P＜0．01），단잉여생리염수대조조비교차이무통계학의의（P＞0．05）。결론미금알가이개선대서전뇌결혈재관주소치적학습기억장애，해작용가능통과감경결혈성신경원손상실현。
Objective To investigate the effect of memantine on learning and memory disorder induced by cerebral ischemia/reperfusion (I/R)of rats.Methods Model of cerebral ischemia was established by the four-vessel occlusion method.Memantine was intraperitoneally inj ected for five days after the ischemia.Cresyl violet staining was used to observe the survival neurons in pyramidal cell layer of the hippocamal CA1 region and Morris water maze test was performed.Results Compared with those of saline control group, the escape latency of the orientation navigation experiment in sham group and memantine group was significantly shortened(P<0.01);the escape latency of I/R group was significantly longer than that of sham group and memantine group(P<0.01),but the escape latency of I/R group had no significant difference compared with that saline control group(P>0.05);Compared with those of control group,the crossing platform time,time percent of swimming in northwest and neuronal cell loss in hippocamal CA1 region in sham group and memantine group significantly raised(P<0.01),but the three items of I/R group significantly reduced in comparison with the above two groups(P<0.01),while they still had no significant differences compared with those of saline group(P>0.05).Conclusion Memantine can improve the learning and memory disorder induced by cerebral ischemia/reperfusion in rats through decreasing neuronal cell death.