浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2014年
20期
1677-1680
,共4页
阎家骏%沈翀%孙泽宇%任煜%周毅%李俊龙%唐桂良%潘建刚%潘寿华%徐国强
閻傢駿%瀋翀%孫澤宇%任煜%週毅%李俊龍%唐桂良%潘建剛%潘壽華%徐國彊
염가준%침충%손택우%임욱%주의%리준룡%당계량%반건강%반수화%서국강
miRNA%膀胱癌%肿瘤标志物
miRNA%膀胱癌%腫瘤標誌物
miRNA%방광암%종류표지물
MiRNA%Bladder cancer%Tumor biomarker
目的：研究膀胱癌患者尿沉渣中miR-142-3p和miR-145-5p差异性表达，为临床寻找早期膀胱癌肿瘤标志物提供依据。方法搜集51例未接受经尿道肿瘤切除术的早期膀胱癌患者和38例健康对照者的清晨中后段尿液，从尿沉渣中提取RNA，采用miRNA特异性茎环逆转录荧光定量PCR检测目标miRNA表达水平，分析两组的受试者工作曲线（ROC），比较miR-142-3p和miR-145-5p及其组合在膀胱癌早期诊断中的价值。结果 miRNA-142-3p在早期膀胱癌患者尿沉渣中显著升高（FC=3.93， P=0.0002，AUC=0.736）。组合测定miR-145-5p/miR-142-3p比值在膀胱癌患者与健康对照者间有统计学差异（P=0.00004， AUC=0.755）。结论测定尿沉渣中的miR-142-3p能有效发现早期膀胱癌，而检测miR-145-5p/miR-142-3p比值的诊断价值更高。
目的：研究膀胱癌患者尿沉渣中miR-142-3p和miR-145-5p差異性錶達，為臨床尋找早期膀胱癌腫瘤標誌物提供依據。方法搜集51例未接受經尿道腫瘤切除術的早期膀胱癌患者和38例健康對照者的清晨中後段尿液，從尿沉渣中提取RNA，採用miRNA特異性莖環逆轉錄熒光定量PCR檢測目標miRNA錶達水平，分析兩組的受試者工作麯線（ROC），比較miR-142-3p和miR-145-5p及其組閤在膀胱癌早期診斷中的價值。結果 miRNA-142-3p在早期膀胱癌患者尿沉渣中顯著升高（FC=3.93， P=0.0002，AUC=0.736）。組閤測定miR-145-5p/miR-142-3p比值在膀胱癌患者與健康對照者間有統計學差異（P=0.00004， AUC=0.755）。結論測定尿沉渣中的miR-142-3p能有效髮現早期膀胱癌，而檢測miR-145-5p/miR-142-3p比值的診斷價值更高。
목적：연구방광암환자뇨침사중miR-142-3p화miR-145-5p차이성표체，위림상심조조기방광암종류표지물제공의거。방법수집51례미접수경뇨도종류절제술적조기방광암환자화38례건강대조자적청신중후단뇨액，종뇨침사중제취RNA，채용miRNA특이성경배역전록형광정량PCR검측목표miRNA표체수평，분석량조적수시자공작곡선（ROC），비교miR-142-3p화miR-145-5p급기조합재방광암조기진단중적개치。결과 miRNA-142-3p재조기방광암환자뇨침사중현저승고（FC=3.93， P=0.0002，AUC=0.736）。조합측정miR-145-5p/miR-142-3p비치재방광암환자여건강대조자간유통계학차이（P=0.00004， AUC=0.755）。결론측정뇨침사중적miR-142-3p능유효발현조기방광암，이검측miR-145-5p/miR-142-3p비치적진단개치경고。
Objective To investigate the contents of miR- 142- 3p and miR- 145- 5p in urine sediment of patients with bladder cancer. Methods Morning mid- stream urine samples were col ected from 51 patients with early stage bladder cancer before transurethral resection and 38 healthy controls. RNAs were extracted from urine sediment and targeting miRNAs were quantified by stem- loop qPCR. The area under receiver operating characteristic curve (AUC- ROC) was used to assess the di-agnostic value of miRNAs for bladder cancer. Results miR- 142- 3p in urine sediment was significantly elevated in urinary sediment of bladder cancer patients (FC=3.93, P=0.0002, AUC=0.736), while the miR- 145- 5p had a trend of reduction (FC=0.59, P=0.056). The ratio of miR- 145- 5p/miR- 142- 3p was significantly lower in early bladder cancer patients than that in healthy controls (FC=0.15, P=0.00004, AUC=0.755). Conclusion MiR- 142- 3p in urine sediment is increased in patients with early bladder cancer, and the ratio of miR- 145- 5p/miR- 142- 3p may be used for early detection of bladder cancer.
