CAJ | 학술논문

目的：探讨少弱精子症患者与正常人精液精子中PRM2基因mRNA的表达水平。方法选取少弱精子症患者20例为实验组，精子正常者10例为对照组，比较两组间精子浓度、活力和PRM2 mRNA水平的变化。结果 PRM2 mRNA水平，实验组与对照组比较差异有统计学意义(P<0.05)；精子浓度，两组间差异有显著统计学意义(P<0.01)；A级精子，两组间比较差异有显著统计学意义(P<0.01)；A+B级精子，两组间差异有显著统计学意义(P<0.01)；另外PRM2 mRNA水平与A级精子和A+B级之间存在正相关性(相关系数分别为0.419，0.571；P值分别为0.021，0.001)。结论少弱精子症患者其PRM2基因mRNA表达水平低于正常人，我们推测PRM2基因的mRNA的表达水平可以在一定程度上反映患者的精子质量水平，检测精子中PRM2 mRNA的表达水平能在一定程度上反映睾丸精子发生过程中的表达事件，可成为男性不育症病因研究、检测和临床治疗效果观察的一个重要指标。
목적：탐토소약정자증환자여정상인정액정자중PRM2기인mRNA적표체수평。방법선취소약정자증환자20례위실험조，정자정상자10례위대조조，비교량조간정자농도、활력화PRM2 mRNA수평적변화。결과 PRM2 mRNA수평，실험조여대조조비교차이유통계학의의(P<0.05)；정자농도，량조간차이유현저통계학의의(P<0.01)；A급정자，량조간비교차이유현저통계학의의(P<0.01)；A+B급정자，량조간차이유현저통계학의의(P<0.01)；령외PRM2 mRNA수평여A급정자화A+B급지간존재정상관성(상관계수분별위0.419，0.571；P치분별위0.021，0.001)。결론소약정자증환자기PRM2기인mRNA표체수평저우정상인，아문추측PRM2기인적mRNA적표체수평가이재일정정도상반영환자적정자질량수평，검측정자중PRM2 mRNA적표체수평능재일정정도상반영고환정자발생과정중적표체사건，가성위남성불육증병인연구、검측화림상치료효과관찰적일개중요지표。
Objective To investigate the expression levels of PRM2 mRNA in oligoasthenozoospermia pa-tients and healthy men with normal semen sperm. Methods Twenty patients with oligoasthenozoospermia were cho-sen as experimental group and 10 healthy men with normal semen sperm were chosen as control group. Then the sperm concentration, vitality and PRM2 mRNA levels between the two groups were compared. Results There were statistical differences between experimental group and controlled group in the expression levels of PRM2 mRNA, sperm concentration, the percentage of grade A sperm and the percentage of grade A+B sperm (P<0.05). And there was a positive correlation between PRM2 mRNA levels and the percentage of grade A sperm and the percentage of grade A+B sperm (correlation coefficients were 0.419 and 0.571, respectively;P were 0.021 and 0.001, respectively). Conclusion The expression level of PRM2 mRNA may predict the patient's sperm quality in a certain extent. Since PRM2 mRNA levels could reflect the expression events in spermatogenesis, it is supposed to be an important indicator in the research of etiology, testing and clinical observation of treatment effect in male infertility.