CAJ | 학술논문

目的：探讨聚合酶链式反应(Polymerase chain reaction，PCR)与DNA测序技术鉴定脓肿分枝杆菌的效果。方法利用细菌16S rRNA基因通用引物PCR扩增疑似分枝杆菌的16S rRNA基因的DNA片段并进行DNA测序分析。DNA测序获得序列经生物信息学比对分析获得相关分枝杆菌的信息；针对相关分枝杆菌分别设计非同源区域特异性引物对1500 bp的克隆DNA片段扩增验证。结果细菌16S rRNA基因通用引物扩增获得约1500 bp的DNA片段，但DNA测序仅获得其中部分DNA序列约296 bp；部分DNA序列经生物信息学比对分析后获得四种同源性高达98%的分枝杆菌信息，并且四种分枝杆菌的特异性引物对1500 bp的克隆产物进行扩增后仅在脓肿分枝杆菌中获得特异性PCR产物。结论疑似结核患者体内的抗酸染色阳性菌为非结核分枝杆菌中的脓肿结核分枝杆菌，并且PCR直接测序法可快速鉴定细菌的种属。
목적：탐토취합매련식반응(Polymerase chain reaction，PCR)여DNA측서기술감정농종분지간균적효과。방법이용세균16S rRNA기인통용인물PCR확증의사분지간균적16S rRNA기인적DNA편단병진행DNA측서분석。DNA측서획득서렬경생물신식학비대분석획득상관분지간균적신식；침대상관분지간균분별설계비동원구역특이성인물대1500 bp적극륭DNA편단확증험증。결과세균16S rRNA기인통용인물확증획득약1500 bp적DNA편단，단DNA측서부획득기중부분DNA서렬약296 bp；부분DNA서렬경생물신식학비대분석후획득사충동원성고체98%적분지간균신식，병차사충분지간균적특이성인물대1500 bp적극륭산물진행확증후부재농종분지간균중획득특이성PCR산물。결론의사결핵환자체내적항산염색양성균위비결핵분지간균중적농종결핵분지간균，병차PCR직접측서법가쾌속감정세균적충속。
Objective To explore the identification of Mycobacterium abscessus with PCR, direct sequencing approaches and bioinformatics. Methods DNA fragments of 16S rRNA gene were acquired using PCR and DNA se-quence obtained by DNA sequencing. DNA about Mycobacterium was analyzed by bioinformatics. Specific primers of non-homologous region for relevant branches Bacillus were designed respectively to clone DNA fragment of 1 500 bp product. Results DNA fragment of 1 500 bp was acquired with PCR, but only a part of the DNA sequence of approx-imately 296 bp was obtained. After DNA sequence bioinformatics analysis, four kind of Mycobacterium with homolo-gy of up to 98%were obtained. Finally, we only obtained PCR special product in M. abscessus when four kinds of spe-cific primers for Mycobacterium were used to clone the DNA fragment of 1 500 bp. Conclusion The Mycobacterium in the suspected TB patients is M. abscessus, and species of bacteria can be identified rapidly by PCR and direct se-quencing approaches.