CAJ | 학술논문

目的：观察去细胞神经支架联合血管内皮生长因子(VEGF)在促进周围神经损伤修复中的作用。方法购买雄性Wistar大鼠60只，先取15只成年Wistar大鼠作为神经供体，制备去细胞神经支架，将制备好的神经支架剪取长度约为1 cm的去细胞神经基膜管。根据实验要求，将余Wistar大鼠随机分为三组，每组大鼠均15只。实验组(A组)为去细胞神经支架移植+局部注射VEGF组；对照组Ⅰ(B组)为去细胞神经支架移植组；对照组Ⅱ(C组)为自体神经移植组。术后喂养1个月，并每日观察大鼠步态、患足溃疡恢复情况。术后1个月取移植段坐骨神经组织行HE染色、免疫组化染色观察再生神经S-100及轴突数量，髓鞘、神经纤维数等生长情况。结果术后1个月，三组大鼠的足部溃疡、步态均有不同程度的恢复，其中实验组恢复率接近于自体神经移植组，明显优于单纯去细胞神经支架移植组；A组的再生神经轴突数为(125.34±5.67)，B组再生神经轴突数为(62.32±3.71)，C组再生神经轴突数为(132.55±7.48)，A组与C组比较差异无统计学意义(P>0.05)，A组与B组比较差异有统计学意义(P<0.05)。结论去细胞神经支架联合VEGF在促进周围神经损伤修复过程中可能具有一定的作用。
목적：관찰거세포신경지가연합혈관내피생장인자(VEGF)재촉진주위신경손상수복중적작용。방법구매웅성Wistar대서60지，선취15지성년Wistar대서작위신경공체，제비거세포신경지가，장제비호적신경지가전취장도약위1 cm적거세포신경기막관。근거실험요구，장여Wistar대서수궤분위삼조，매조대서균15지。실험조(A조)위거세포신경지가이식+국부주사VEGF조；대조조Ⅰ(B조)위거세포신경지가이식조；대조조Ⅱ(C조)위자체신경이식조。술후위양1개월，병매일관찰대서보태、환족궤양회복정황。술후1개월취이식단좌골신경조직행HE염색、면역조화염색관찰재생신경S-100급축돌수량，수초、신경섬유수등생장정황。결과술후1개월，삼조대서적족부궤양、보태균유불동정도적회복，기중실험조회복솔접근우자체신경이식조，명현우우단순거세포신경지가이식조；A조적재생신경축돌수위(125.34±5.67)，B조재생신경축돌수위(62.32±3.71)，C조재생신경축돌수위(132.55±7.48)，A조여C조비교차이무통계학의의(P>0.05)，A조여B조비교차이유통계학의의(P<0.05)。결론거세포신경지가연합VEGF재촉진주위신경손상수복과정중가능구유일정적작용。
Objective To investigate the effect of VEGF in combination with acellular nerve scaffold on re-pairing peripheral nerve defect. Methods Sixty male Wistar rats were bought, 15 of which were selected as nerve do-nors. The acellular nerve scaffolds were prepared and then cut into 1 cm acellular nerve basal laminas. According to ex-perimental design, the rest 45 rats were randomly divided into three groups, with 15 in each group. Group A (the experi-mental group) was treated with both VEGF and acellular nerve scaffolds transplantation, group B (control groupⅠ) with just acellular nerve scaffolds transplantation, group C (control groupⅡ) with nerve autograft. After surgery, the re-covery of the rats' gait and plantar ulcer were observed every day. After one month of the surgery, the regenerative nerves' S-100 protein, number of axon, growth of myelin sheath and nerve fiber were observed by HE staining and im-munohistochemical staining of the transplanted sciatic nerve tissue. Results One month after the surgery, the three groups showed different recovery levels of the rats' gait and plantar ulcer. The recovery rate of group A was close to that of group C, but significantly higher than that of group B. The number of regenerative nerve axon of group A, B and C was (125.34±5.67), (62.32±3.71), and (132.55±7.48), respectively. They were no statistically significant difference be-tween group A and group C (P>0.05), while the difference between group A and group B was statistically significant (P<0.05). Conclusion VEGF in combination with allogenic acellular nerve can help repairing peripheral nerve defect.