CAJ | 학술논문

目的：探讨体外分离培养糖尿病患者脂肪间充质干细胞（ADSCs）的方法并分析其生物学特性。方法采用胶原酶消化法从糖尿病患者的脂肪组织中分离出ADSCs。对ADSCs进行体外培养，倒置相差显微镜下观察细胞形态；绘制第3、6、8代传代细胞的生长曲线；流式细胞仪检测第3代细胞表面标记物（ CD34、CD45、CD29、CD44、CD105、CD90）；通过成脂诱导实验鉴定细胞的分化潜能（油红染色阳性判定为脂肪细胞，证实细胞可向脂肪细胞分化）。结果分离出的ADSCs，能稳定传代；其细胞表面标记物CD34、CD45呈阴性表达，CD29、CD90、CD44、CD105呈阳性表达。成脂诱导后油红染色阳性。结论在糖尿病患者脂肪组织中成功分离到ADSCs，在体外适宜条件下ADSCs可向脂肪细胞分化，具有分化潜能。
목적：탐토체외분리배양당뇨병환자지방간충질간세포（ADSCs）적방법병분석기생물학특성。방법채용효원매소화법종당뇨병환자적지방조직중분리출ADSCs。대ADSCs진행체외배양，도치상차현미경하관찰세포형태；회제제3、6、8대전대세포적생장곡선；류식세포의검측제3대세포표면표기물（ CD34、CD45、CD29、CD44、CD105、CD90）；통과성지유도실험감정세포적분화잠능（유홍염색양성판정위지방세포，증실세포가향지방세포분화）。결과분리출적ADSCs，능은정전대；기세포표면표기물CD34、CD45정음성표체，CD29、CD90、CD44、CD105정양성표체。성지유도후유홍염색양성。결론재당뇨병환자지방조직중성공분리도ADSCs，재체외괄의조건하ADSCs가향지방세포분화，구유분화잠능。
Objective To establish the isolation and culture method of adipose mesenchymal stem cells derived from diabetic patients in vitro, so as to observe biological properties.Methods The ADSCs were isolated by digestion with col-lagenase from adipose tissue of diabetic patients, then cultured and purified in vitro.The cells were observed by inverted microscopeand.The P3, P6, P8 generation cells were collected and analyzed the cell growth curve.Then the cell surface markers and karyotype analysis of the P3 generation cells were detected.Their differentiation potential was proved by adipo-genic differentiation.Oil red staining positive proved they were fat cells.Results After isolation and culture, we obtained the ADSCs, which could passage stably and proliferate highly.The flow cytometry examination showed that the expression of CD34 and CD45 were negative, CD29 , CD90 , CD44 and CD105 were positive.Oil red staining was positive.Conclusion The ADSCs could be isolated from diabetic patients adipose tissue and be functionally induced into adipocytes in the pres-ence of appropriate conditioned media.