农业科技与装备
農業科技與裝備
농업과기여장비
AGRICULTURAL SCIENCE & TECHNOLOGY AND EQUIPMENT
2014年
9期
9-12
,共4页
辛夷%顺序提取物%灰葡萄孢菌%抑制作用
辛夷%順序提取物%灰葡萄孢菌%抑製作用
신이%순서제취물%회포도포균%억제작용
Violet magnolia%gradational extracts%Bocrytis Cinerea Pers.%inhibition effect
采用顺序提取法，分别用石油醚、氯仿、乙酸乙酯、丙酮、乙醇、水6种溶剂对辛夷进行提取。结果表明：去离子水的提取率最大，为12.06%；石油醚次之，为9.63%。采用生长速率法和孢子萌发法分别测定了辛夷6种顺序提取物对黄瓜灰霉病致病菌的菌丝生长和孢子萌发的抑制效果。结果表明：辛夷6种顺序提取物对灰葡萄孢菌的菌丝生长和孢子萌发均有抑制作用，且对菌丝生长的 EC50基本小于1000 mg/L，对孢子萌发的 EC50均在1300 mg/L 以上，说明辛夷各溶剂提取物对菌丝生长的抑制效果均优于孢子萌发；石油醚、氯仿和去离子水提取物对菌丝生长和孢子萌发的 EC50分别为372.15 mg/L 和1318.92 mg/L，229.84 mg/L 和1965.50 mg/L 以及845.44 mg/L 和4321.80 mg/L。
採用順序提取法，分彆用石油醚、氯倣、乙痠乙酯、丙酮、乙醇、水6種溶劑對辛夷進行提取。結果錶明：去離子水的提取率最大，為12.06%；石油醚次之，為9.63%。採用生長速率法和孢子萌髮法分彆測定瞭辛夷6種順序提取物對黃瓜灰黴病緻病菌的菌絲生長和孢子萌髮的抑製效果。結果錶明：辛夷6種順序提取物對灰葡萄孢菌的菌絲生長和孢子萌髮均有抑製作用，且對菌絲生長的 EC50基本小于1000 mg/L，對孢子萌髮的 EC50均在1300 mg/L 以上，說明辛夷各溶劑提取物對菌絲生長的抑製效果均優于孢子萌髮；石油醚、氯倣和去離子水提取物對菌絲生長和孢子萌髮的 EC50分彆為372.15 mg/L 和1318.92 mg/L，229.84 mg/L 和1965.50 mg/L 以及845.44 mg/L 和4321.80 mg/L。
채용순서제취법，분별용석유미、록방、을산을지、병동、을순、수6충용제대신이진행제취。결과표명：거리자수적제취솔최대，위12.06%；석유미차지，위9.63%。채용생장속솔법화포자맹발법분별측정료신이6충순서제취물대황과회매병치병균적균사생장화포자맹발적억제효과。결과표명：신이6충순서제취물대회포도포균적균사생장화포자맹발균유억제작용，차대균사생장적 EC50기본소우1000 mg/L，대포자맹발적 EC50균재1300 mg/L 이상，설명신이각용제제취물대균사생장적억제효과균우우포자맹발；석유미、록방화거리자수제취물대균사생장화포자맹발적 EC50분별위372.15 mg/L 화1318.92 mg/L，229.84 mg/L 화1965.50 mg/L 이급845.44 mg/L 화4321.80 mg/L。
Using petroleum ether, chloroform, ethyl acetate, acetone, ethanol, deionized water to gradational extract violet magnolia. Result showed that the extracted rate of deionized water is the largest,which is 12.06%. The next is petroleum ether which is 9.63%. The inhibitory effects of 6 kinds violet magnolia (petroleum ether, chloroform, ethyl acetate, acetone, water) gradational extracts on hyphae growth and spore germination of bocrytis Cinerea Pers. pathogen were studied with growth rate and spores germination methods. Results showed that 6 kinds solvent gradational extracts had significant antibacterial effects on hyphae growth and spore germination of pathogen. The EC50 of 6 kinds solvent gradational extracts on hyphae growth is lower than 1 000 mg/L, which is higher than 1 300 mg/L on spore germination. Indicate that the inhibited effect on hyphae growth was superior to spore germination of 6 kinds solvent gradational extracts. The EC50 of petroleum ether, chloroform and deionized water extracts on hyphae growth and spore germination is 372.15 mg/L and 1318.92 mg/L, 229.84 mg/L and 1 965.50 mg/L, 845.44 mg/L and 4 321.80 mg/L respectively.
