CAJ | 학술논문

目的：研究凋亡促进因子Omi/HtrA2基因过表达对食管癌细胞EC9706凋亡及对耐药性的影响。方法用脂质体包裹转染人食管癌EC9706细胞，实验分为对照组、空载体组、Omi载体组、顺铂组、Omi+顺铂联合组，采用RT-PCR检测不同干预组Omi/HtrA2基因mRNA表达的变化，MTT法检测不同干预组细胞生长抑制率，流式细胞术检测不同干预组细胞的凋亡率，分析Omi/HtrA2基因蛋白表达对细胞耐药性的影响。结果①Omi/HtrA2基因mRNA在EC9706细胞中的表达：对照组为（0.113±0.009），空载体组为（0.106±0.006），Omi载体组为（0.436±0.021），顺铂组为（0.196±0.013），Omi+顺铂联合组为（0.639±0.032），顺铂组、Omi+顺铂联合组较对照组、空载体组明显增强，差异有统计学意义（P<0.01，P=0.011，P=0.000），对照组与空载体组比较无显著性差异（P>0.05，P=0.725）。②MTT法检测Omi/HtrA2的表达对细胞生长的抑制率：对照组为0，空载体组为2.89%，Omi载体组为27.61%，顺铂组为30.02%，Omi+顺铂联合组为48.47%，与对照组相比，Omi载体组、顺铂组、Omi+顺铂联合组均显著抑制EC9706细胞的生长，差异具有显著性（P=0.000）。③流式细胞术检测Omi/HtrA2的表达对凋亡的影响：对照组细胞凋亡率为10.76%，空载体组为13.26%，Omi载体组为38.14%，顺铂组为45.86%，Omi载体+顺铂联合组为56.64%，与对照组、空载体组比较，Omi载体组、顺铂组、Omi载体+顺铂联合干预组细胞凋亡率明显提高，差异具有显著性意义（P=0.000）。结论 Omi/HtrA2基因过表达可以明显抑制癌细胞的生长，提高癌细胞的凋亡率及癌细胞对化疗药物的敏感性，且Omi/HtrA2基因的表达与化疗药物具有协同抗癌作用。
목적：연구조망촉진인자Omi/HtrA2기인과표체대식관암세포EC9706조망급대내약성적영향。방법용지질체포과전염인식관암EC9706세포，실험분위대조조、공재체조、Omi재체조、순박조、Omi+순박연합조，채용RT-PCR검측불동간예조Omi/HtrA2기인mRNA표체적변화，MTT법검측불동간예조세포생장억제솔，류식세포술검측불동간예조세포적조망솔，분석Omi/HtrA2기인단백표체대세포내약성적영향。결과①Omi/HtrA2기인mRNA재EC9706세포중적표체：대조조위（0.113±0.009），공재체조위（0.106±0.006），Omi재체조위（0.436±0.021），순박조위（0.196±0.013），Omi+순박연합조위（0.639±0.032），순박조、Omi+순박연합조교대조조、공재체조명현증강，차이유통계학의의（P<0.01，P=0.011，P=0.000），대조조여공재체조비교무현저성차이（P>0.05，P=0.725）。②MTT법검측Omi/HtrA2적표체대세포생장적억제솔：대조조위0，공재체조위2.89%，Omi재체조위27.61%，순박조위30.02%，Omi+순박연합조위48.47%，여대조조상비，Omi재체조、순박조、Omi+순박연합조균현저억제EC9706세포적생장，차이구유현저성（P=0.000）。③류식세포술검측Omi/HtrA2적표체대조망적영향：대조조세포조망솔위10.76%，공재체조위13.26%，Omi재체조위38.14%，순박조위45.86%，Omi재체+순박연합조위56.64%，여대조조、공재체조비교，Omi재체조、순박조、Omi재체+순박연합간예조세포조망솔명현제고，차이구유현저성의의（P=0.000）。결론 Omi/HtrA2기인과표체가이명현억제암세포적생장，제고암세포적조망솔급암세포대화료약물적민감성，차Omi/HtrA2기인적표체여화료약물구유협동항암작용。
Objective To study the influence of Omi/HtrA2 expression on apoptosis of esophageal cancer cell EC9706 and drug resistance. Methods Used liposome mediated method to wrap transfecting human esophageal cancer cell EC9706. There were control group,empty vector group,Omi carrier group,cisplatin treated group and Omi+cisplatin combination group in the study. For all the intervention groups,the mRNA expression levels of Omi/HtrA2 gene were detected by RT-PCR,the inhibi-tion rates of cell growth were detected by MTT method,the apoptosis rates were measured by flow cytometry,and the influence of Omi/HtrA2 protein expression on the drug resistance of cells was also analyzed. Results ①The mRNA expression of Omi/HtrA2 gene in EC9706 cells were as the followings:control group (0.113±0.009),empty vector group (0.106±0.006),Omi transected group (0.436±0.021),cisplatin treated group (0.196±0.013),Omi+cisplatin combination group (0.639±0.032). Com-pared with the control group and empty vector group,the mRNA expression levels of Omi/HtrA2 gene in EC9706 cells in cis-platin treated group and Omi+cisplatin combination group had significantly increased and the differences were statistically signif-icant (P<0.01,P=0.011,P=0.000),whereas the control group and empty vector group showed no significant difference (P>0.05, P=0.725). ②The inhibition rates of Omi/HtrA2 expression on the cell growth by MTT method were as followings: control group was 0,empty vector group was 2.89%,Omi transected group was 27.61%,cisplatin treated group was 30.02%,Omi+cisplatin combination group was 48.47%. Compared with the control group ,the expression of Omi/HtrA2 significantly inhibited the cell growth in Omi transected group,cisplatin treated group and Omi+cisplatin combination group,the difference were significant (P=0.000). ③The influence of Omi/HtrA2 expression on the apoptosis were detected by flow cytometry,the apoptosis rates in all groups were as followings after different interventions:the control group was 10.76%,empty vector group was 13.26%,Omi tran-sected group was 38.14%,cisplatin treated group was 45.86%,Omi+cisplatin combination group was 56.64%. Compared with the control group and empty vector group,the apoptosis rates in the other three groups were all increased significantly and the differences were significant (P=0.000). Conclusion The expression of Omi/HtrA2 can significantly inhibit the growth of esophageal cancer cells,enhance the apoptosis rate of cancer cells and the sensitivity of cancer cells to chemotherapy drugs,and the expression of Omi/HtrA2 gene has synergistic anti-cancer effect with chemotherapy drugs.