CAJ | 학술논문

目的：将双重PCR与毛细管电泳技术相结合，建立一种验证转录组测序结果的新方法。方法根据前期进行转录组测序的分析结果，挑选8个差异基因作为靶基因，分别使用双重PCR结合琼脂糖凝胶电泳、双重PCR结合毛细管电泳以及实时荧光定量PCR进行验证，比较三者的验证率。结果双重PCR结合琼脂糖凝胶电泳的验证率为50％；双重PCR结合毛细管电泳和实时荧光定量PCR的验证率均为100％。结论双重PCR结合毛细管电泳可作为转录组测序结果验证的一种有效方法。
목적：장쌍중PCR여모세관전영기술상결합，건립일충험증전록조측서결과적신방법。방법근거전기진행전록조측서적분석결과，도선8개차이기인작위파기인，분별사용쌍중PCR결합경지당응효전영、쌍중PCR결합모세관전영이급실시형광정량PCR진행험증，비교삼자적험증솔。결과쌍중PCR결합경지당응효전영적험증솔위50％；쌍중PCR결합모세관전영화실시형광정량PCR적험증솔균위100％。결론쌍중PCR결합모세관전영가작위전록조측서결과험증적일충유효방법。
Objective To establish a method to validate the result of transcriptome sequencing using duplex PCR tech -nology combined with capillary electrophoresis .Methods According to a previous study on transcriptome sequencing , eight differentially expressed genes were chosen as target genes for examination .The mRNA expression level of these genes was detected using duplex PCR combined with agarose gel electrophoresis , duplex PCR combined with capillary electropho-resis and Q-PCR, respectively.Then, the verification efficiency of each method was evaluated carefully .Results The ver-ification efficiency of duplex PCR combined with agarose gel electrophoresis was 50%, while that of duplex PCR combined with capillary electrophoresis and Q-PCR was both 100%.Conclusion Combination of duplex PCR technology with capil-lary electrophoresis can be used as an alternative method to validate the results of transcriptome sequencing .