农业灾害研究
農業災害研究
농업재해연구
JOURNAL of AGRICULTURAL CATASTROPHOLOGY
2014年
9期
4-5
,共2页
赤腹松鼠%物种识别%Cytb
赤腹鬆鼠%物種識彆%Cytb
적복송서%물충식별%Cytb
Callosciurus erythraeus%Species identification%Cyt b
为确定在大别山地区采集到的一个Callosciurus属松鼠样本的物种归属及分类地位,利用分子遗传学手段的对所采样本进行研究。采用Cyt b基因结合PCR技术、测序技术及BLAST技术分析样本的遗传信息,再通过MEGA建树确定样本的分类地位。BLAST分析结果显示该物种的遗传信息与赤腹松鼠序列相似程度最高,而建树结果显示与赤腹松鼠聚为同一支,从而确定该物种应为赤腹松鼠,属哺乳纲、啮齿目、松鼠科、丽松鼠属。
為確定在大彆山地區採集到的一箇Callosciurus屬鬆鼠樣本的物種歸屬及分類地位,利用分子遺傳學手段的對所採樣本進行研究。採用Cyt b基因結閤PCR技術、測序技術及BLAST技術分析樣本的遺傳信息,再通過MEGA建樹確定樣本的分類地位。BLAST分析結果顯示該物種的遺傳信息與赤腹鬆鼠序列相似程度最高,而建樹結果顯示與赤腹鬆鼠聚為同一支,從而確定該物種應為赤腹鬆鼠,屬哺乳綱、齧齒目、鬆鼠科、麗鬆鼠屬。
위학정재대별산지구채집도적일개Callosciurus속송서양본적물충귀속급분류지위,이용분자유전학수단적대소채양본진행연구。채용Cyt b기인결합PCR기술、측서기술급BLAST기술분석양본적유전신식,재통과MEGA건수학정양본적분류지위。BLAST분석결과현시해물충적유전신식여적복송서서렬상사정도최고,이건수결과현시여적복송서취위동일지,종이학정해물충응위적복송서,속포유강、교치목、송서과、려송서속。
In order to determine the species attribution and classification sta-tus of a Callosciurus genus sample of squirrel collected in Dabie Mountain area, the sample was studied by molecu-lar genetic approaches. The genetic in-formation of the sample was analyzed using Cyt b gene combined with PCR technology, sequencing technology and BLAST technology, and then the classifi-cation status of the sample was deter-mined using phylogenetic tree construct-ed by MEGA. BLAST analysis results showed that the genetic information of this species had the highest sequence similarity with Callosciurus erythraeus, and the phylogenetic tree construction results showed that the sample was clus-tered into the same team with Callosciu-rus erythraeus, then it was determined that this species should belong to the Callosciurus, Sciuridae,c Rodentia, Mam-malia.