医学临床研究
醫學臨床研究
의학림상연구
JOURNAL OF CLINICAL RESEARCH
2014年
10期
1949-1951,1952
,共4页
黎建先%蔡林%陈勇
黎建先%蔡林%陳勇
려건선%채림%진용
低温%脑出血/代谢%NF-κB/代谢%细胞黏附分子/代谢%疾病模型, 动物%大鼠, Spra-gue-Dawley
低溫%腦齣血/代謝%NF-κB/代謝%細胞黏附分子/代謝%疾病模型, 動物%大鼠, Spra-gue-Dawley
저온%뇌출혈/대사%NF-κB/대사%세포점부분자/대사%질병모형, 동물%대서, Spra-gue-Dawley
Hypothermia%Cerebral Hemorrhage/ME%NF-kappa B/ME%Cell Adhesion Molecules/ME%Disease Models,Animal%Rats,Sprague-Dawley
【目的】研究亚低温对大鼠急性脑出血（intracerebral hemorrhage ，ICH）后脑组织含水量及核转录因子（nuclear factor‐κB ，NF‐κB）和细胞间黏附分子1（Intercellular adhesion molecule‐1，ICAM‐1）表达的影响。【方法】将50只健康雄性SD大鼠，随机（随机数字法）分为假手术组（SO）、常温组（NT ）、亚低温组1 h组（HT1）、亚低温组2 h组（HT2）和亚低温组4 h组（HT4），每组10只大鼠，采用大鼠基底节注入自体不凝血法制作脑出血模型。SO组和NT组大鼠控制体温在（37．0±0．3）℃；亚低温组在脑出血后迅速降温至（33．0±0．4）℃并分别保持1 h、2 h和4 h。检测各组大鼠脑组织含水量，凝胶电泳迁移率实验（EMSA ）检测 NF‐κB活性；Western blot 检测ICAM‐1蛋白的表达。【结果】与SO组相比较，NT组大鼠脑组织含水量显著升高，NF‐κB活性及ICAM‐1蛋白表达均显著降低，其差异有统计学意义（ P ＜0．05）；NT 组与 HT1、HT2和 HT4组大鼠脑含水量、NF‐κB活性及ICAM‐1蛋白表达比较均有显著性差异（ P ＜0．05）；HT2组和 HT4组相比较，大鼠脑含水量、NF‐κB活性及ICAM‐1蛋白表达比较均无统计学意义（ P ＞0．05）。【结论】早期亚低温治疗可抑制大鼠脑出血后NF‐κB活性和IC A M‐1蛋白的表达，从而减轻脑水肿。
【目的】研究亞低溫對大鼠急性腦齣血（intracerebral hemorrhage ，ICH）後腦組織含水量及覈轉錄因子（nuclear factor‐κB ，NF‐κB）和細胞間黏附分子1（Intercellular adhesion molecule‐1，ICAM‐1）錶達的影響。【方法】將50隻健康雄性SD大鼠，隨機（隨機數字法）分為假手術組（SO）、常溫組（NT ）、亞低溫組1 h組（HT1）、亞低溫組2 h組（HT2）和亞低溫組4 h組（HT4），每組10隻大鼠，採用大鼠基底節註入自體不凝血法製作腦齣血模型。SO組和NT組大鼠控製體溫在（37．0±0．3）℃；亞低溫組在腦齣血後迅速降溫至（33．0±0．4）℃併分彆保持1 h、2 h和4 h。檢測各組大鼠腦組織含水量，凝膠電泳遷移率實驗（EMSA ）檢測 NF‐κB活性；Western blot 檢測ICAM‐1蛋白的錶達。【結果】與SO組相比較，NT組大鼠腦組織含水量顯著升高，NF‐κB活性及ICAM‐1蛋白錶達均顯著降低，其差異有統計學意義（ P ＜0．05）；NT 組與 HT1、HT2和 HT4組大鼠腦含水量、NF‐κB活性及ICAM‐1蛋白錶達比較均有顯著性差異（ P ＜0．05）；HT2組和 HT4組相比較，大鼠腦含水量、NF‐κB活性及ICAM‐1蛋白錶達比較均無統計學意義（ P ＞0．05）。【結論】早期亞低溫治療可抑製大鼠腦齣血後NF‐κB活性和IC A M‐1蛋白的錶達，從而減輕腦水腫。
【목적】연구아저온대대서급성뇌출혈（intracerebral hemorrhage ，ICH）후뇌조직함수량급핵전록인자（nuclear factor‐κB ，NF‐κB）화세포간점부분자1（Intercellular adhesion molecule‐1，ICAM‐1）표체적영향。【방법】장50지건강웅성SD대서，수궤（수궤수자법）분위가수술조（SO）、상온조（NT ）、아저온조1 h조（HT1）、아저온조2 h조（HT2）화아저온조4 h조（HT4），매조10지대서，채용대서기저절주입자체불응혈법제작뇌출혈모형。SO조화NT조대서공제체온재（37．0±0．3）℃；아저온조재뇌출혈후신속강온지（33．0±0．4）℃병분별보지1 h、2 h화4 h。검측각조대서뇌조직함수량，응효전영천이솔실험（EMSA ）검측 NF‐κB활성；Western blot 검측ICAM‐1단백적표체。【결과】여SO조상비교，NT조대서뇌조직함수량현저승고，NF‐κB활성급ICAM‐1단백표체균현저강저，기차이유통계학의의（ P ＜0．05）；NT 조여 HT1、HT2화 HT4조대서뇌함수량、NF‐κB활성급ICAM‐1단백표체비교균유현저성차이（ P ＜0．05）；HT2조화 HT4조상비교，대서뇌함수량、NF‐κB활성급ICAM‐1단백표체비교균무통계학의의（ P ＞0．05）。【결론】조기아저온치료가억제대서뇌출혈후NF‐κB활성화IC A M‐1단백적표체，종이감경뇌수종。
[Objective] To explore the effect of mild hypothermia on water content ,nuclear factor‐B(NF‐κB) and intercellular adhesion molecule‐1 (ICAM‐1 ) in brain tissue of rats after intracerebral hemorrhage (ICH ) .[Methods] Totally 50 healthy male Sprague‐Dawley(SD) rats were randomly(random number method) divided in‐to sham‐operated group(SO group) ,normothermic group(NT group) ,1h‐mild‐hypothermia group(HT1 group) , 2h‐mild‐hypothermia group(HT2 group) and 4h‐mild‐hypothermia group(HT4 group) with 10 rats in each group . ICH model was established by stereotactic infusion of autologous blood into caudate nucleus .Rats in NT group and SO group were kept at (37 .0 ± 0 .3)℃ of body temperature .Rats in HT groups after ICH were kept at (33 .0 ± 0 .4) ℃ of body temperature for 1 ,2 and 4h ,respectively .Water content in brain tissue of rats in each group was measured .NF‐κB activity was detected by electrophoretic mobility shift assay (EMSA) .The expression of ICAM‐1 was detected by Western blot .[Results] Compared with SO group ,brain water content and the expression of NF‐κB and ICAM‐1 in NT group were markedly increased ,and there was significant difference( P<0 .05) .Brain water content and the expression of NF‐κB and ICAM‐1 in HT1 ,HT2 and HT4 groups were significantly lower than those in NT group( P<0 .05) .There was no significant difference in brain water content and the expression of NF‐κB and ICAM‐1 between HT2 group and HT4 group( P>0 .05) .[Conclusion]Early mild hypothermia can inhibit the expression of NF‐κB and ICAM‐1 in rats after intracerebral hemorrhage ,and alleviate brain edema .
