CAJ | 학술논문

以昆明小鼠为受试动物,随机分为6组,包括1个阴性对照组、3个氯氰菊酯染毒组、1个维生素E组和1个高剂量氯氰菊酯加维生素E组,染毒组按10,20,40mg/kg水平,维生素E的剂量为100mg/kg,灌胃染毒小鼠7d.以肾组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)和8-羟基脱氧鸟苷(8-OHdG)的含量；以肾组织细胞测定 DNA-蛋白质交联(DPC)系数.随着氯氰菊酯染毒剂量的升高,肾组织的ROS、MDA、8-OHdG含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量为20mg/kg 时,MDA 和8-OHdG 含量差异有统计学意义(P<0.05)；染毒剂量为40mg/kg 时, ROS(F=3.7044)、GSH(F=3.4908)、MDA(F=3.5851)、8-OHdG含量(F=11.7934)和DPC系数(F=6.9165)差异均有统计学意义(P<0.05, P<0.01).病理学观察可见20,40mg/kg剂量组小鼠肾小球增生肥大,肾小管上皮细胞水肿,管腔变小.与高剂量染毒组相比较,高剂量染毒加维生素E组肾组织的ROS、MDA含量、8-OHdG含量和DPC系数均有下降,GSH含量上升(P<0.05, P<0.01).高剂量(≥20mg/kg)的氯氰菊酯能造成小鼠肾组织的氧化损伤,维生素E有抗氧化作用.
이곤명소서위수시동물,수궤분위6조,포괄1개음성대조조、3개록청국지염독조、1개유생소E조화1개고제량록청국지가유생소E조,염독조안10,20,40mg/kg수평,유생소E적제량위100mg/kg,관위염독소서7d.이신조직균장측정활성양(ROS)、환원형곡광감태(GSH)、병이철(MDA)화8-간기탈양조감(8-OHdG)적함량；이신조직세포측정 DNA-단백질교련(DPC)계수.수착록청국지염독제량적승고,신조직적ROS、MDA、8-OHdG함량화DPC계수축점상승,GSH함량축점강저,각지표정일정적제량-효응관계.염독제량위20mg/kg 시,MDA 화8-OHdG 함량차이유통계학의의(P<0.05)；염독제량위40mg/kg 시, ROS(F=3.7044)、GSH(F=3.4908)、MDA(F=3.5851)、8-OHdG함량(F=11.7934)화DPC계수(F=6.9165)차이균유통계학의의(P<0.05, P<0.01).병이학관찰가견20,40mg/kg제량조소서신소구증생비대,신소관상피세포수종,관강변소.여고제량염독조상비교,고제량염독가유생소E조신조직적ROS、MDA함량、8-OHdG함량화DPC계수균유하강,GSH함량상승(P<0.05, P<0.01).고제량(≥20mg/kg)적록청국지능조성소서신조직적양화손상,유생소E유항양화작용.
Kunming mice were randomly grouped into six groups and treated with orally administered drugsona daily base for a week. The groups included one solvent control group, three cypermethrin groups, one high dose cypermethrin plus vitamin E protection group and one vitamin E group. The exposure doses of cypermethrin groups were 10, 20 and 40mg/kg respectively. Some kidney tissues were then made into homogenates for the measurement of reactive oxygen species (ROS), glutathione (GSH), 8-hydroxy-2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) contents. Meanwhile, DNA-protein crosslink (DPC) coefficients were detected from brain cell suspension.The kidney contents of ROS, MDA, 8-OHdG and DPC coefficients increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. In the exposure group with the dose of 20mg/kg, MDA contents and DPC coefficients were significantly higher than those of the control group (P<0.05); ROS (F=3.7044), GSH (F=3.4908), MDA (F=3.5851), 8-OhdG (F=11.7934) and DPC (F=6.9165) levels were significantly different in levels of each biomarker between 40mg/kg group and control group (P<0.05,P<0.01). Furthermore, the high-dosaged (20and 40mg/kg) group had increased glomerulus cells, proliferation and hypertrophy of glomerulus, hydrops of renal tubular epithelial cell, and narrow lumen. Administration of vitamin E (VE) to cypermethrin-treated mice reflected that ROS, MDA, 8-OHdG and DPC increased whereas GSH decreased (P<0.05,P<0.01). Cyermethrin at certain doses (≥20mg/kg) could induce oxidative stress in mice kidney, whereas vitamin E had antioxidant effects.