CAJ | 학술논문

采用高通量测序技术,对南海南部海域浮游细菌丰度、群落组成和群落多样性的分布特征及与环境因子的关系进行了研究.结果表明,该研究区域浮游细菌丰度为107~108个/L,近岸大于离岸,同一站位不同水层细菌分布差异明显.优势类群为变形菌门、蓝藻门和拟杆菌门,优势亚群为γ-变形菌纲、α-变形菌纲、蓝藻菌纲和黄杆菌纲,研究区域内不同水体间物种组成存在较大差异,另外该海域还存在大量未被认知的细菌类群.该海域浮游细菌种类丰富,具有较高的多样性指数(H′)(4.44~7.00),研究区域内表层水体H′接近,分别为5.26、5.33和5.07,处于上升流的次表层水体中H′为6.70明显高于其他水层.DOC和磷酸盐是影响该海域浮游细菌丰度的主要因素,同时磷酸盐也是影响其群落多样性的主要因素,表明该海域异养浮游细菌生长主要受P的限制.
채용고통량측서기술,대남해남부해역부유세균봉도、군락조성화군락다양성적분포특정급여배경인자적관계진행료연구.결과표명,해연구구역부유세균봉도위107~108개/L,근안대우리안,동일참위불동수층세균분포차이명현.우세류군위변형균문、람조문화의간균문,우세아군위γ-변형균강、α-변형균강、람조균강화황간균강,연구구역내불동수체간물충조성존재교대차이,령외해해역환존재대량미피인지적세균류군.해해역부유세균충류봉부,구유교고적다양성지수(H′)(4.44~7.00),연구구역내표층수체H′접근,분별위5.26、5.33화5.07,처우상승류적차표층수체중H′위6.70명현고우기타수층.DOC화린산염시영향해해역부유세균봉도적주요인소,동시린산염야시영향기군락다양성적주요인소,표명해해역이양부유세균생장주요수P적한제.
The high-throughput sequencing approach was adopted to analyze the distribution characteristics of the abundance, community structure and community diversity of bacterioplankton in the southern South China Sea and their relationship with environmental factors. The results indicated that the bacterioplankton abundance in the investigated area was about 107~108cells/L with high values in offshore stations and sharp differences among water layers. The dominant groups were Proteobacteria, Cyanobacteria and Bacteroidetes and the dominant classes were γ-Proteobacteria,α-Proteobacteria, Cyanobacterium and Flavobacteria. The community structure of bacterioplankton showed an obvious difference among various water bodies and numerous unidentified bacteria were recorded. The bacterioplankton showed a high species richness in the area with a biodiversity index (H') of between 4.44~7.00. The biodiversity index in surface layers of the area was 5.26, 5.33 and 5.07, respectively, whereas the index in the upwelling subsurface layer was 6.70, which was significantly higher than other layers. DOC and phosphate were the main factors influencing the bacterioplankton abundance, phosphate was also the main factor affecting the community diversity. The growth of bacterioplankton in the southern South China Sea was therefore mainly limited by phosphate.