CAJ | 학술논문

探讨了在腐殖质模式物蒽醌?2?磺酸钠(AQS)存在条件下,奥奈达希瓦氏菌MR-1的还原U(VI)特性.结果表明,在厌氧环境下奥奈达希瓦氏菌以AQS为电子穿梭载体,利用电子供体高效还原U(VI).当菌体投加量为1.2×109个时,其还原铀的效率达95.09%；AQS的浓度低于0.5mmol/L时有利于MR-1菌厌氧还原U(VI),AQS浓度的升高U(VI)的还原明显受到抑制.当U(VI)初始浓度为30.0mg/L时,分别以甲酸盐、乙酸盐和乳酸盐为电子供体,经过7d后其还原率分别达到95.37%、92.41%和95.65%.金属离子(Cu2+、Mn2+、Ca2+)、有毒有机物等对U(VI)还原产生影响.当Ca2+的浓度为2.0mmol/L时,对U(VI)的还原有微弱的促进作用,而当Cu2+和Mn2+浓度为2.0mmol/L时,则存在较强的抑制作用.奥奈达希瓦氏菌也能利用环境中甲苯、三氯乙酸、顺丁烯二酸等有毒物质高效还原U(VI),同时使有毒物质得到降解.扫描电子显微镜(SEM)和电子能谱(EDS)分析结果表明,奥奈达希瓦氏菌菌体中沉积了铀元素.
탐토료재부식질모식물은곤?2?광산납(AQS)존재조건하,오내체희와씨균MR-1적환원U(VI)특성.결과표명,재염양배경하오내체희와씨균이AQS위전자천사재체,이용전자공체고효환원U(VI).당균체투가량위1.2×109개시,기환원유적효솔체95.09%；AQS적농도저우0.5mmol/L시유리우MR-1균염양환원U(VI),AQS농도적승고U(VI)적환원명현수도억제.당U(VI)초시농도위30.0mg/L시,분별이갑산염、을산염화유산염위전자공체,경과7d후기환원솔분별체도95.37%、92.41%화95.65%.금속리자(Cu2+、Mn2+、Ca2+)、유독유궤물등대U(VI)환원산생영향.당Ca2+적농도위2.0mmol/L시,대U(VI)적환원유미약적촉진작용,이당Cu2+화Mn2+농도위2.0mmol/L시,칙존재교강적억제작용.오내체희와씨균야능이용배경중갑분、삼록을산、순정희이산등유독물질고효환원U(VI),동시사유독물질득도강해.소묘전자현미경(SEM)화전자능보(EDS)분석결과표명,오내체희와씨균균체중침적료유원소.
Characteristics and reaction conditions of anaerobic reduction by the Shewanella oneidensis MR-1in the presence of anthraquinone-2-sulfonate (AQS) were evaluated. The results showed that U(VI) could be efficiently reduced by S. oneidensis MR-1utilizing AQS and electron donors under the anaerobic conditions. Its reduction efficiency reached 95.09%when the dosage of MR-1was 1.2×109. The efficiency of U(VI) bioreduction increased when the concentration of AQS was below 0.5mmol/L, and inhibited when it exceeded 0.5mmol/L. When the initial concentration of U (VI) was 30mg/L, uranium reduction rates were 95.37%, 92.41%and 95.65%while using formate, acetate and lactate respectively. Metal ions (Cu2+, Mn2+, Ca2+) and toxic organics had impacts on the reduction of U (VI). Ca2+acted as a weak role in promoting the reduction, however, equal concentrations of Cu2+and Mn2+played a strong inhibitory effect. Toxic organic compounds were available to reduce U (VI) efficiently by S. oneidensis MR-1and get degraded at the same time. Characterizations with Scanning Electron Microscope (SEM) and Energy dispersive spectroscopy (EDS) indicated the deposition of U element on the cell of S. oneidensis.