CAJ | 학술논문

采用紫外-可见吸收光谱、1H-核磁共振(NMR)和同步荧光光谱,研究了牛粪堆肥水溶性有机物(DOM)的结构特征及其演化规律.紫外-可见吸收光谱分析显示,SUVA254由堆肥起始的1.161上升至堆肥结束的2.543；E465/E665在整个堆肥过程中呈现上升趋势,变化范围在2.333~3.500；260~280,460~480,600~700nm 范围内的面积积分 A1、A2和 A3均呈现出先增大后减小的趋势,峰值分别出现在26,14,14d.1H-NMR分析显示,堆肥0d到堆肥41d,0.5~3.1δ面积积分所占比例从43.06%下降至8.63%,3.1~5.5δ从56.07%上升至89.68%,5.5~10δ不足总体的6%且变化趋势不明显.同步荧光光谱结果显示,经过41d 的堆肥,蛋白质类物质区积分面积比例(APLR)由0.331下降到0.252,而富里酸类物质区积分面积比例(AFLR)由0.325增加到0.336,同时胡敏酸类物质区积分面积比例(AHLR)由0.344增加到0.412；AFLR/APLR比与同步荧光光谱3个荧光峰峰高存在显著相关性,AHLR/AFLR与两个荧光峰光强的比值I351/I284和I382/I351存在显著相关性.上述结果表明,随着堆肥进行, DOM 中的非腐殖质物质转化为类腐殖质,同时其芳香性结构增多,碳链结构发生氧化反应,分子量从小变大,堆肥腐殖化程度加大,稳定度增加.
채용자외-가견흡수광보、1H-핵자공진(NMR)화동보형광광보,연구료우분퇴비수용성유궤물(DOM)적결구특정급기연화규률.자외-가견흡수광보분석현시,SUVA254유퇴비기시적1.161상승지퇴비결속적2.543；E465/E665재정개퇴비과정중정현상승추세,변화범위재2.333~3.500；260~280,460~480,600~700nm 범위내적면적적분 A1、A2화 A3균정현출선증대후감소적추세,봉치분별출현재26,14,14d.1H-NMR분석현시,퇴비0d도퇴비41d,0.5~3.1δ면적적분소점비례종43.06%하강지8.63%,3.1~5.5δ종56.07%상승지89.68%,5.5~10δ불족총체적6%차변화추세불명현.동보형광광보결과현시,경과41d 적퇴비,단백질류물질구적분면적비례(APLR)유0.331하강도0.252,이부리산류물질구적분면적비례(AFLR)유0.325증가도0.336,동시호민산류물질구적분면적비례(AHLR)유0.344증가도0.412；AFLR/APLR비여동보형광광보3개형광봉봉고존재현저상관성,AHLR/AFLR여량개형광봉광강적비치I351/I284화I382/I351존재현저상관성.상술결과표명,수착퇴비진행, DOM 중적비부식질물질전화위류부식질,동시기방향성결구증다,탄련결구발생양화반응,분자량종소변대,퇴비부식화정도가대,은정도증가.
UV-visible spectra, 1H-nuclear magnetic resonance (NMR) and synchronous-scan fluorescence spectra were applied to investigate the structural characteristics and evolution of dissolved organic matter (DOM) from cattle manure during the process of composting. The result from UV-visible spectra showed that, SUVA254 increased from 1.1614 at the initial stage to 2.543 at the curing stage, E465/E665 exhibited an upward trend with a variation range of 2.333~3.500, and the integration area within the range of 260~280 (A1), 460~480 (A2) and 600~700 nm (A3) all increased first then decreased with the maximum values at 26,14 in 14d, respectively. The result from the 1H-NMR analysis showed that, percentage of the integration area within the range of 0.5~3.1δdecreased from 43.06%to 8.63%, 3.1~5.5δincreased from 56.07% to 89.68%, and 5.5~10δ was below 6% with a insignificant variation tendency during composting. The analysis from synchronous-scan fluorescence spectra showed that, the integrated area ratio of protein-like substances (APLR) decreased from 0.331 to 0.252, that of fulvic-like acid matter (AFLR) increased from 0.325 to 0.336, while that of humic-like acid matter (AHLR) increased from 0.344 to 0.412 at the same time. The AFLR/APLR ratio and the fluorescence intensities of three peaks from synchronous-scan spectra exhibited an obvious correlation. In addition, significant correlation also observed between AHLR/AFLR ratio and I351/I284 and I382/I351 ratios. of DOM increased, the carbon structure In conclusion non-humic substances were transformed into humic matter during cattle manure composting, the aromaticity chains were oxidized, and the molecular weight of DOM increased, which increased the humification degree and stability of composts.