CAJ | 학술논문

目的：探讨MsrA在结直肠癌干细胞中的表达及与结直肠癌发生、发展的关系。方法免疫磁珠分选结直肠癌细胞株SW480细胞中CD133+/CD44+/ESA+亚群细胞，RT-PCR检测癌细胞、癌干细胞和正常大肠粘膜细胞中MsrA的表达以及MsrA过表达对VEGF、MMP-13和CXCR4表达的影响，MTT检测过表达MsrA对结直肠癌干细胞增殖的影响。结果癌干细胞中MsrA的表达水平明显高于癌细胞，癌细胞中MsrA的表达水平明显高于正常大肠粘膜细胞。MsrA过表达会抑制结直肠癌干细胞的增殖以及下调VEGF、MMP-13和CXCR4的表达。结论 MsrA可能通过下调VEGF、MMP-13和CXCR4的表达和抑制细胞增殖来抑制结直肠癌的发生、发展。
목적：탐토MsrA재결직장암간세포중적표체급여결직장암발생、발전적관계。방법면역자주분선결직장암세포주SW480세포중CD133+/CD44+/ESA+아군세포，RT-PCR검측암세포、암간세포화정상대장점막세포중MsrA적표체이급MsrA과표체대VEGF、MMP-13화CXCR4표체적영향，MTT검측과표체MsrA대결직장암간세포증식적영향。결과암간세포중MsrA적표체수평명현고우암세포，암세포중MsrA적표체수평명현고우정상대장점막세포。MsrA과표체회억제결직장암간세포적증식이급하조VEGF、MMP-13화CXCR4적표체。결론 MsrA가능통과하조VEGF、MMP-13화CXCR4적표체화억제세포증식래억제결직장암적발생、발전。
Objective To investigate the expression of methionine sulfoxide reductase (MsrA) in colorectal cancer stem cells and its association with the tumorigenesis and progression of colorectal cancer. Methods The CD133+/CD44+/ESA+subpopulation of colorectal cancer cell line SW480 was obtained by magnetic activated cell sorting (MACS). The expression of MsrA, VEGF, MMP-13 and CXCR4 in the cancer cells, cancer stem cells and normal colon mucosa cells were detected using RT-PCR. The proliferation of colorectal cancer stem cells was evaluated with MTT assay. Results The expression of MsrA was significantly higher in cancer stem cells than in the cancer cells and normal mucosa cells. Overexpression of MsrA inhibited the proliferation of colorectal cancer stem cells and down-regulated the expression of VEGF, MMP-13 and CXCR4. Conclusions MsrA suppresses the tumorigenesis and progression of colorectal cancer cells possibly by inhibiting cell proliferation and down-regulating VEGF, MMP-13 and CXCR4.