CAJ | 학술논문

目的：观察复方黄甘对5/6肾切除慢性肾衰模型大鼠的疗效，并探讨其可能的作用机制。方法用HPLC检测复方黄甘提取物组分并定量；构建5/6肾切除大鼠慢性肾衰模型，将模型大鼠随机分为尿毒清组、科素亚组、复方黄甘低、中、高剂量组和模型组，另加假手术组共7组，每组10只大鼠，经灌胃给药12周后用全自动生化分析仪检测Scr、BUN、Ucr及24 h UPr；对残余肾组织进行HE和Masson染色观察病理变化；用RT-PCR和免疫组化检测肾组织FN、MCP-1、ICAM-1的表达。结果复方黄甘提取物中主要成分及其含量为：甘草苷（0.06%）、丹皮酚（1.06%）、芦荟大黄素（0.1%）、大黄酸（0.47%）、大黄素（0.19%）、大黄酚（0.41%）和大黄素甲醚（0.13%）；复方黄甘低、中、高剂量组Scr、BUN、Ucr、Ccr及24h UPr都显著低于模型组（P<0.05）；复方黄甘给药组肾小球硬化和肾间质纤维化改善较为明显，且肾组织中FN、ICAM-1 mRNA表达和蛋白表达均显著低于模型组（P<0.05）。结论复方黄甘可改善慢性肾衰模型大鼠肾功能，减轻肾小球硬化和肾间质纤维化，在一定程度上延缓慢性肾功能衰竭的进展。
목적：관찰복방황감대5/6신절제만성신쇠모형대서적료효，병탐토기가능적작용궤제。방법용HPLC검측복방황감제취물조분병정량；구건5/6신절제대서만성신쇠모형，장모형대서수궤분위뇨독청조、과소아조、복방황감저、중、고제량조화모형조，령가가수술조공7조，매조10지대서，경관위급약12주후용전자동생화분석의검측Scr、BUN、Ucr급24 h UPr；대잔여신조직진행HE화Masson염색관찰병리변화；용RT-PCR화면역조화검측신조직FN、MCP-1、ICAM-1적표체。결과복방황감제취물중주요성분급기함량위：감초감（0.06%）、단피분（1.06%）、호회대황소（0.1%）、대황산（0.47%）、대황소（0.19%）、대황분（0.41%）화대황소갑미（0.13%）；복방황감저、중、고제량조Scr、BUN、Ucr、Ccr급24h UPr도현저저우모형조（P<0.05）；복방황감급약조신소구경화화신간질섬유화개선교위명현，차신조직중FN、ICAM-1 mRNA표체화단백표체균현저저우모형조（P<0.05）。결론복방황감가개선만성신쇠모형대서신공능，감경신소구경화화신간질섬유화，재일정정도상연완만성신공능쇠갈적진전。
Objective To observe the effect of compound Huang Gan in delaying chronic renal failure in rats after 5/6 nephrectomy and explore the possible mechanisms. Methods High-performance liquid chromatography was used to was used identify the components of compound Huang Gan extract. Rat models of 5/6 nephrectomy received a 12-week treatment with intragastric administration of Niaoduqing, Cozaar, or compound Huang Gan at low, moderate or high doses (n=10). After the treatments, the rats were sacrificed for detecting Scr, BUN, Ucr and 24h UPr , pathological examination of the renal tissues, and determination of FN, MCP-1, and ICAM-1 expression levels in the renal tissues using RT-PCR and immunohistochemistry. Results The major chemical components of compound Huang Gan extract included glycyrrhizin (0.61%), paeonol (1.2%), aloe emodin (0.72%), rhein (0.85%), emodin (0.87%), chrysophanol (0.79%) and physcion (0.8%). Treatment with compound Huang Gan at low, moderate and high doses significantly reduced Scr, BUN, Ucr , Ccr and 24 h UPr levels (P<0.05), improved interstitial fibrosis and glomerulosclerosis, and reduced FN and ICAM-1 expressions (P<0.05) in rats following nephrectomy. Conclusion Compound Huang Gan can improve the renal function and lessen glomerulosclerosis and renal interstitial fibrosis to delay the progression of chronic renal failure in rat models of 5/6 nephrectomy.