CAJ | 학술논문

目的：探讨电针对非酒精性脂肪性肝病大鼠肝组织toll样受体4（TLR4）、核因子κB（NF-κB）表达的影响及意义。方法48只雄性SD大鼠随机分成正常组、模型组、早期干预组、晚期干预组，每组各12只；模型组、早期干预组、晚期干预组均给予高脂饲料喂养5周建立非酒精性脂肪性肝病模型，在5周末处死每组大鼠各1只，以验证造模成功。早期干预组和晚期干预组均电针“足三里”“、丰隆”“、三阴交”，1次/d，20 min/次，连续治疗21 d。针刺后检测各组大鼠血清ALT、AST、TC、TG的变化情况，采用荧光定量PCR和Werstern blot检测大鼠肝脏TLR4和NF-κB的mRNA表达情况。结果模型组大鼠的ALT、AST、TC、TG及肝脏TLR4、NF-κB的表达均高于其余3组，差异有统计学意义（P<0.05）；针刺后各干预组的ALT、AST、TC、TG及肝脏TLR4、NF-κB的表达均显著下降，差异有统计学意义（P<0.05）。结论电针“足三里”“、丰隆”“、三阴交”可以调节血脂及肝功，对非酒精性脂肪肝有一定良性调节作用，其作用机制可能与电针可以调节脂代谢，同时可以下调肝脏组织TLR4、NF-κB的表达，减轻炎症对非酒精性脂肪性肝病的影响有关。
목적：탐토전침대비주정성지방성간병대서간조직toll양수체4（TLR4）、핵인자κB（NF-κB）표체적영향급의의。방법48지웅성SD대서수궤분성정상조、모형조、조기간예조、만기간예조，매조각12지；모형조、조기간예조、만기간예조균급여고지사료위양5주건립비주정성지방성간병모형，재5주말처사매조대서각1지，이험증조모성공。조기간예조화만기간예조균전침“족삼리”“、봉륭”“、삼음교”，1차/d，20 min/차，련속치료21 d。침자후검측각조대서혈청ALT、AST、TC、TG적변화정황，채용형광정량PCR화Werstern blot검측대서간장TLR4화NF-κB적mRNA표체정황。결과모형조대서적ALT、AST、TC、TG급간장TLR4、NF-κB적표체균고우기여3조，차이유통계학의의（P<0.05）；침자후각간예조적ALT、AST、TC、TG급간장TLR4、NF-κB적표체균현저하강，차이유통계학의의（P<0.05）。결론전침“족삼리”“、봉륭”“、삼음교”가이조절혈지급간공，대비주정성지방간유일정량성조절작용，기작용궤제가능여전침가이조절지대사，동시가이하조간장조직TLR4、NF-κB적표체，감경염증대비주정성지방성간병적영향유관。
Objective To investigate the effect of electro-acupuncture on Toll-like receptor 4 (TLR4) nuclear factor κB (NF-κB) expressions in the liver of rats with non-alcoholic fatty liver disease (NAFLD). Methods SD rat models of NAFLD induced by feeding high-fat diet for 5 weeks were subjected to early or late intervention with electro-acupuncture at the acupoints Zusanli, Fenglong and Sanyinjiao for 20 min once daily for 21 consecutive days. The changes in serum ALT, AST, TC, and TG levels were determined in the rats and hepatic expressions of TLR4 and NF-κB detected with quantitative fluorescence PCR and Western blotting after treatments. Results Serum ALT, AST, TC, and TG levels and TLR4 and NF-κB levels increased significantly after NAFLD onset (P<0.05), and were markedly reduced by interventions with electro-acupuncture (P<0.05). Conclusion Electro-acupuncture at the acupoints Zusanli, Fenglong and Sanyinjiao can modify blood lipid and hepatic function to produce positive effect on NAFLD possibly by modifying lipid metabolism, lowering expressions of TLR4 and NF-κB in the liver, and reducing the impact of inflammation on NAFLD.